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Trypsin sensitivity of mammalian cells grown in a serum-free medium

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Summary

Two mammalian cell lines which multiply in vitro in culture medium devoid of serum were investigated for sensitivity to twice crystallized trypsin. The minimum trypsin concentration which showed a concomitant increase in cell numbers and detachment from the surface of the culture vessel in one cell line was 0.01 μg per ml or approximately 10−8 μg per cell. These effects could be neutralized by normal human or calf serum at a dilution of 1∶500. The second cell line, which normally grows in suspension, was unaffected by these concentrations of trypsin.

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Author notes

  1. William G. Taylor

    Present address: Laboratory of Biology, Tissue Culture Section, National Cancer Institute, 20014, Bethesda, Md

Authors and Affiliations

  1. Department of Microbiology, University of Illinois, College of Medicine, 60680, Chicago, Illinois

    Robert W. Pumper, Peter Fagan &  William G. Taylor

  2. St. James Hospital, Chicago Heights, 60411, Illinois

    Robert W. Pumper, Peter Fagan &  William G. Taylor

Authors
  1. Robert W. Pumper
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  2. Peter Fagan
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  3. William G. Taylor
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Pumper, R.W., Fagan, P. & Taylor, W.G. Trypsin sensitivity of mammalian cells grown in a serum-free medium. In Vitro 6, 266–268 (1971). https://doi.org/10.1007/BF02625939

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  • DOI: https://doi.org/10.1007/BF02625939

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