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Decalcified egg shell membrane, a supporting substrate for electron microscopic cross-sections of monolayers of epithelial cell line MDCK

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Summary

Decalcified chicken egg shell membrane is a suitable physical substrate for the growth of monolayers of an epithelial line of tissue culture cells, MDCK. The shell membranes with their adherent sheets of cells are easily prepared for cross-section study of monolayer growth with electron microscopy.

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Authors and Affiliations

  1. Cancer Bioassay Research Laboratory, Department of Pathology, University of Pittsburgh, 15213, Pittsburgh, Pennsylvania

    Joseph Leighton, Sunder Mansukhani & Larry W. Estes

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  1. Joseph Leighton
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  2. Sunder Mansukhani
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  3. Larry W. Estes
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Additional information

This study was supported by Research Grant P-442 from the American Cancer Society.

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Leighton, J., Mansukhani, S. & Estes, L.W. Decalcified egg shell membrane, a supporting substrate for electron microscopic cross-sections of monolayers of epithelial cell line MDCK. In Vitro 6, 251–252 (1971). https://doi.org/10.1007/BF02625936

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  • DOI: https://doi.org/10.1007/BF02625936

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