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Establishment of two cell lines from the nematodeMeloidogyne incognita (tylenchida; meloidogynidae)

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Summary

The root-knot nematodeMeloidogyne incognita Chitwood, is a pest of considerable agricultural and economic importance. As part of a study of this species, two diploid cell lines (2n=32–36) were establishedin vitro on two different culture media. The two cultures were obtained from mature egg-laying females and were designated MI 8C and MI 5G. Descriptions are given of the techniques used for the preparation of the primary cultures, the media and the characteristics of the cell lines obtained. The doubling time of the population was estimated.

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References

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Authors and Affiliations

  1. Department of Bichemistry, University of Cambridge, Tennis Court Road, CB2 1QW, Cambridge, England

    Thanasis Manousis & David J. Ellar

Authors
  1. Thanasis Manousis
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  2. David J. Ellar
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This work was supported by the A. G. C.

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Manousis, T., Ellar, D.J. Establishment of two cell lines from the nematodeMeloidogyne incognita (tylenchida; meloidogynidae). In Vitro Cell Dev Biol 26, 1105–1114 (1990). https://doi.org/10.1007/BF02624448

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  • DOI: https://doi.org/10.1007/BF02624448

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