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Comparative properties of untreated andN-nitrosobenzylmethyl-amine-transformed rat esophageal epithelial cell lines

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Summary

A culture system utilizing rat esophageal epithelial cells has been developed. Four normal and eightN-nitrosobenzylmethylamine-treated lines were compared with respect to chromosome number, anchorage-independent growth in agarose, and tumorigenic potential in syngenic rats. All cell lines were aneuploid with nine in the near-tetraploid range and three in the near-diploid range. No relation between tumorigenic potential and chromosome number or structure was apparent. Similarly, anchorage-independent growth in agarose did not correlate with tumorigenic potential. Three of the 12 immortalized lines (two carcinogen-treated and 1 untreated) induced well-differentiated squamous cell carcinomas in syngeneic rats. These tumors had weak metastatic potentials suggesting that tumorigenic potential and metastatic ability are separately controlled. These cell lines will be useful for the investigation of factors involved in the conversion of immortalized rat esophageal epithelial cell lines to lines of high metastatic potential.

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Authors and Affiliations

  1. Department of Pathology, Medical College of Ohio, 3000 Arlington Avenue, 43614, Toledo, Ohio

    Gary D. Stoner, William T. Gunning III & Neilma Budd

  2. The Upjohn Company, 49001, Kalamazoo, Michigan

    Merrill S. Babcock

  3. Department of Pediatrics, Medical College of Ohio, Bowling Green State University, 43402, Bowling Green, Ohio

    Maureen M. Mc Corquodale

  4. Department of Medical Technology, Bowling Green State University, 43402, Bowling Green, Ohio

    Roubadeh Jamasbi

  5. Cell Culture Laboratory, Children's Hospital of Michigan, 3901 Beaubien Boulevard, 48201, Detroit, Michigan

    Bharati Hukku

Authors
  1. Gary D. Stoner
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  2. Merrill S. Babcock
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  3. Maureen M. Mc Corquodale
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  4. William T. Gunning III
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  5. Roubadeh Jamasbi
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  6. Neilma Budd
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  7. Bharati Hukku
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Stoner, G.D., Babcock, M.S., Mc Corquodale, M.M. et al. Comparative properties of untreated andN-nitrosobenzylmethyl-amine-transformed rat esophageal epithelial cell lines. In Vitro Cell Dev Biol 25, 899–908 (1989). https://doi.org/10.1007/BF02624002

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  • DOI: https://doi.org/10.1007/BF02624002

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