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Maintenance and characterization of human myometrial smooth muscle cells in monolayer culture

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Summary

Human myometrial cells were dispersed from uterine tissue by limited enzymatic digestion of myometrium that was obtained at the time of hysterectomy. The dispersed myometrial cells that are obtained in this manner can be maintained in monolayer culture in the presence of medium that contains fetal bovine serum. In primary culture, as well as after passage, the characteristics of these cells are morphologically and biochemically similar to those of smooth muscle cells and myometrial tissue.

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Authors and Affiliations

  1. Cecil H. and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical School, 75235, Dallas, Texas

    M. Linette Casey, Paul C. MacDonald, Murray D. Mitchell & Jeanne M. Snyder

  2. Department of Biochemistry, The University of Texas Southwestern Medical School, 75235, Dallas, Texas

    M. Linette Casey, Paul C. MacDonald, Murray D. Mitchell & Jeanne M. Snyder

  3. Department of Obstetrics and Gynecology, The University of Texas Southwestern Medical School, 75235, Dallas, Texas

    M. Linette Casey, Paul C. MacDonald, Murray D. Mitchell & Jeanne M. Snyder

  4. Department of Cell Biology, The University of Texas Southwestern Medical School, 75235, Dallas, Texas

    M. Linette Casey, Paul C. MacDonald, Murray D. Mitchell & Jeanne M. Snyder

Authors
  1. M. Linette Casey
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  2. Paul C. MacDonald
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  3. Murray D. Mitchell
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  4. Jeanne M. Snyder
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Additional information

This investigation was supported, in part, by U.S. Public Health Service Grants 5-P50-HD11149 and 5-P01-AG00306.

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Casey, M.L., MacDonald, P.C., Mitchell, M.D. et al. Maintenance and characterization of human myometrial smooth muscle cells in monolayer culture. In Vitro 20, 396–403 (1984). https://doi.org/10.1007/BF02619585

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  • DOI: https://doi.org/10.1007/BF02619585

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