Summary
Cultured rat Schwann cells do not exhibit the ring-like changes in cell shape previously reported to be induced in the Schwann cell line RN22 by elevation of intracellular cyclic AMP. They do, however, undergo different shape changes on treatment with cholera toxin or low serum concentration. Furthermore, DNA synthesis in the cell line is inhibited by treatment with cholera toxin and unaffected by bovine pituitary extract, though both of these agents stimulate DNA synthesis in normal Schwann cells. Our results, therefore, do not support the hypothesis that elevation of intracellular cyclic AMP is a positive signal for myelination by the Schwann cell. Moreover, they illustrate the need for caution in drawing conclusions about normal cells of the nervous system from studies on neural cell lines.
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References
Brockes, J. P., K. L. Fields, and M. C. Raff. 1977. A surface antigenic marker for rat Schwann cells. Nature 266: 364–366.
Brockes, J. P., K. L. Fields, and M. C. Raff. 1979. Studies on cultured rat Schwann cells. I. Derivation of purified populations from cultures of peripheral nerve. Brain Res. 165: 105–118.
Raff, M. C., A. Hornby-Smith, and J. P. Brockes. 1978. Cyclic AMP as a mitogenic signal for cultured rat Schwann cells. Nature 273: 672–673.
Pfeiffer, S. E., and W. Wechsler 1972. Biochemically differentiated neoplastic clone of Schwann cells. Proc. Nat. Acad. Sci. U.S.A. 69: 2885–2889.
Shepphard, J. R., T. H. Hudson, and J. R. Larson. 1975. Adenosine 3′,5′-monophosphate analogs promote a circular morphology of cultured Schwannoma cells. Science 187: 179–181.
Raff, M. C., E. R. Abney, J. P. Brockes, and A. Hornby-Smith. 1978. Schwann cell growth factors. Cell 15: 813–822.
Bennett, V., and P. Cuatrecasas. 1977. The specificity and action of animal, bacterial and plant toxins. In: P. Cuatrecasas (Ed.),Receptors and Recognition, Series B. Vol. 1: 3–86.
Schubert, D., S. Heinemann, W. Carlisle, B. Kimes, J. Patrick, J. H. Steinbach, W. Culp, and B. L. Brandt. 1974. Clonal cell lines from the rat central nervous system. Nature 249: 224–227.
Asbury, A. L. 1967. Schwann cell proliferation in developing mouse sciatic nerve. J. Cell Biol. 34: 735–743.
Folkman, J., and A. Moscona. 1978. Role of cell shape in growth control. Nature 273: 345–349.
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Paper I in this series is reference 2.
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Brockes, J.P., Raff, M.C. Studies on cultured rat Schwann cells. In Vitro 15, 772–778 (1979). https://doi.org/10.1007/BF02618303
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DOI: https://doi.org/10.1007/BF02618303