Summary
Cultures of embryonic rat brain and liver, and embryonic turkey brain were inoculated with sporozoites ofPlasmodium berghei. Sporozoites succeeded in establishing exoerythrocytic infections in approximately 10% of the cultures. The exoerythrocytic parasites developed to a late schizont stage with some showing early segmentation although free merozoites were not observed. The morphology and rate of development of the exoerythrocytic parasites in culture appear similar to that seen in vivo.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Davis, A. G., C. G. Huff, and T. T. Palmer. 1966. Procedures for maximum production of exoerythrocytic stages ofPlasmodium fallax in tissue cultures. Exp. Parasitol. 19: 1–8.
Beaudoin, R. L., and C. P. A. Strome. 1970. Cultivation of exoerythrocytic stages of malaria (abstr.). J. Parasitol. 56: 26.
Beaudoin, R. L., C. P. A. Strome, and W. G. Clutter. 1974. Cultivation of avian malaria parasites in mammalian liver cells. Exp. Parasitol. 36: 355–359.
Beaudoin, R. L., C. P. A. Strome, and W. G. Clutter. 1969. A tissue culture system for the study of drug action against the tissue phase of malaria. Mil. Med. 134: 979–985.
Beaudoin, R. L. 1977. Should cultivated exoerthrocytic parasites be considered as a source of antigen for a malaria vaccine. Bull. W.H.O. 55: 373–376.
Foley, D. A., J. Kennard, and J. P. Vanderberg. 1978.Plasmodium berghei: Infective exoerythrocytic schizonts in primary monolayer cultures of rat liver cells. Exp. Parasitol. 46: 166–178.
Doby, J. M., and R. Barker. 1976. Essais d'obtentionin vitro des formes pré-érythrocytaires dePlasmodium vivax en cultures de cellules hépatiques humaines inoculées par sporozoites. C.R. Soc. Biol. 170: 661–665.
Yoeli, M., and H. Most. 1965. Studies on sporozoite-induced infections of rodent malaria. I. The pre-erythrocytic tissue stage ofPlasmodium berghei. Am. J. Trop. Med. Hyg. 14: 700–714.
McGhee, R. B. 1950. The ability of the avian malaria parasite,Plasmodium lophurae, to infect erythrocytes of distantly related species of animals. Am. J. Hyg. 52: 42–47.
Author information
Authors and Affiliations
Additional information
This work was supported by ONR Contract No. N00014-76-C-1132 and Naval Medical Research and Development Command, Research Work Unit No. M0095PN.002.5058. the opinions and assertions contained herein are the private ones of the authors and are not to be construed as official or reflecting the views of the Navy Department or the naval service at large. The experiments reported herein were conducted according to the principles set forth in theGuide for the Care and Use of Laboratory Animals, Institute of Laboratory Resources, National Research Council, DHEW, Pub. No. (NIH) 74-23.
Rights and permissions
About this article
Cite this article
Strome, C.P.A., De Santis, P.L. & Beaudoin, R.L. The cultivation of the exoerythrocytic stages ofPlasmodium berghei from sporozoites. In Vitro 15, 531–536 (1979). https://doi.org/10.1007/BF02618155
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02618155