Summary
Studies of acid phosphates produced by cell lines MA 160 and EB 33 demonstrated immunochemically their prostatic origin. MA 160 and EB 33, rather than being HeLa contaminants, may be hybrids of prostatic epithelial and HeLa cells or true prostatic cell lines with chromosomal changes common to all long-term cultivated cell lines.
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References
Fraley, E. E., S. Ecker, and M. M. Vincent. 1970. Spontaneous in vitro neoplastic transformation of adult human prostatic epithelium. Science 170: 540–542.
Okada, K., and F. H. Schroder. 1974. Human prostatic carcinoma in cell culture: Preliminary report on development and characterization of an epithelial cell line (EB 33). Urol. Res. 2: 111–121.
Nelson-Rees, W. A., R. R. Flandermeyer, and P. K. Hawthorne. 1974. Banded marker chromosomes as indicators of intraspecies cellular contamination. Science 184: 1093–1096.
Nelson-Rees, W. A., and R. R. Flandermeyer. 1976. HeLa cultures defined. Science 191: 96–98.
Webber, M. M., P. K. Horan, and T. R. Bouldin. 1977. Present status of MA-160 cell line, prostatic epithelium or HeLa cells? Invest. Urol. 14: 335–343.
Okada, K., I. Laudenbach, and F. H. Schroeder. 1976. Human prostatic epithelial cells in culture: Clonal selection and androgenic dependence of cell line EB 33. J. Urol. 115: 164–167.
Ofner, P., R. L. Vena, N. J. Barowsky, R. M. Singer, and A. H. Tashjian, Jr. 1977. Comparative C19-radiosteroid metabolism by MA 160 and HeLa cell lines. In Vitro 13: 378–388.
Chang, R. S. 1977. HeLa marker chromosomes, Chang liver cells and liver-specific functions. Science 199: 567.
Nelson-Rees, W. A. 1977. HeLa marker chromosomes, Chang liver cells, and liver-specific functions (comment). Science 199: 567–568.
Choe, B. K., E. J. Pontes, I. McDonald, and N. R. Rose. 1977. Immunochemical studies of prostatic acid phosphatase. Cancer Treat. Rep. 61: 201–204.
Pontes, J. E., B. Choe, N. Rose, and J. M. Pierce Jr. 1977. Indirect immunofluorescence for identification of prostatic epithelial cells. J. Urol. 117: 459–463.
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This research was supported by NIH (Cancer) Research Grants Nos. 18748 and 16426; and Detroit General Hospital Research Corporation.
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Pontes, J.E., Pierce, J.M., Choe, BK. et al. MA 160 and EB 33 cell lines: HeLa cell contaminants, hybrids or prostatic epithelial cells?. In Vitro 15, 469–472 (1979). https://doi.org/10.1007/BF02618148
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DOI: https://doi.org/10.1007/BF02618148