Summary
Although each diploid cell within an individual probably contains a full and identical complement of all the DNA species characterizing that individual, not all of these DNA species are transcribed to RNA within any one cell or tissue. Stable epigenetic mechanisms select specific portions of the DNA genome for transcription. Such selective mechanisms are seen to employ a nonspecific generalized repression of all DNA within a cell by means of complex formations with polycationic histone repressors. Nuclear polyanionic de-repressors antagonize the interaction between DNA and histones, and effect a partial displacement of repressor histones from localized portions of the DNA genome. Such histone displacement allows these DNA segments to undergo an intermittent separation of the strands of the DNA double helix. A species of nuclear RNA appears to function as a highly selective agent in de-repression by hybridizing with a single DNA strand, freeing the complementary DNA strand for continuous specific messenger RNA synthesis.
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This investigation was supported in part by Research Career Development Award CA-17857 from the United States Public Health Service.
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Frenster, J.H. Mechanisms of repression and de-repression within interphase chromatin. In Vitro 1, 78–101 (1965). https://doi.org/10.1007/BF02618091
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DOI: https://doi.org/10.1007/BF02618091