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Gamma-irradiated plant callus tissue: Cytokinins from transfer ribonucleic acid

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Summary

Callus tissue ofHaworthia mirabilis Haw. was irradiated with60Co gamma rays. tRNA was isolated, hydrolyzed enzymatically, and cytokinin-active ribonucleosides were separated by Sephadex LH-20 column chromatography and assayed with the tobaccocallus cytokinin bioassay. Three cytokinins were detected in tRNA from irradiated tissue, two of which chromatographed with zeatin riboside and N6-(Δ2-isopentenyl)adenosine. The third cytokinin-active ribonucleoside was retained longer than the above compounds on the Sephadex column and may be 2-methylthio-N6-(Δ2-isopentenyl)adenosine. Two cytokinins were detected in tRNA from nonirradiated tissue—those chromatographed with zeatin riboside and N6-(Δ2-isopentenyl)adenosine. Relationships between cytokinins from tRNA and free cytokinins found in tissue earlier are discussed.

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Authors and Affiliations

  1. Thomas Hunt Morgan School of Biological Sciences, University of Kentucky, 40546, Lexington, Kentucky

    K. N. Pandey & P. S. Sabharwal

  2. College of Agriculture, University of Kentucky, 40546, Lexington, Kentucky

    T. R. Kemp & R. A. Andersen

Authors
  1. K. N. Pandey
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  2. T. R. Kemp
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  3. P. S. Sabharwal
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  4. R. A. Andersen
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This is paper 78-10-124 of the Kentucky Agricultural Experiment Staton and is published with approval of the Director.

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Pandey, K.N., Kemp, T.R., Sabharwal, P.S. et al. Gamma-irradiated plant callus tissue: Cytokinins from transfer ribonucleic acid. In Vitro 15, 857–860 (1979). https://doi.org/10.1007/BF02618040

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  • DOI: https://doi.org/10.1007/BF02618040

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