Skip to main content
SpringerLink
Log in

A method for in situ embedding of cultured cells grown on plastic surfaces

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

A method is described for the electron microscopic visualization of contact relationships between monolayer cells and the supporting surface of the plastic culture vessel.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Egeberg, J. 1965. Sandwich embedding of monolayers of cells in epoxy resin for ultrathin sectioning. Stain Technol. 40: 343–346.

    PubMed  CAS  Google Scholar 

  2. Zagury, D., P. Zeitoun, and M. Viette. 1966. Cytologie: Methode d'étude de cultures de cellules par la microscopic électronique: la culture sur support d'Epon. C. R. Acad. Sci. [D] Paris. 262: 2458–2459.

    Google Scholar 

  3. Zagury, D., G. D. Pappas, and P. I. Marcus. 1968. Preparation of cell monolayers for combined light and electron microscopy: staining in blocks. J. Microscopie 7: 287–291.

    Google Scholar 

  4. Smith, W., E. W. Gray, and J. M. K. Mackay. 1969. A sandwich-embedding technique for monolayers of cells cultured on Araldite. J. Microsc. 89: 359–363.

    PubMed  CAS  Google Scholar 

  5. Abercrombic, M., J. E. M. Heaysman, and S. M. Pegrum. 1971. The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamells. Exp. Cell Res. 67: 359–367.

    Article  Google Scholar 

  6. Flaxman, B. A., M. A. Lutzner, and E. J. Van Scott. 1968. Ultrastructure of cell attachment to substratum in vitro. J. Cell Biol. 36: 406–410.

    Article  PubMed  CAS  Google Scholar 

  7. Dalen, H., and T. J. Nevalainen. 1968. Direct epoxy embedding for vertical sectioning of cells grown as a monolayer on Millipore filter. Strain Technol. 43: 217–220.

    CAS  Google Scholar 

  8. McCombs, R. M., M. Benyesh-Melnick, and J. P. Brunschwig. 1968. The use of Millipore filters in ultrastructure studies of cell cultures and viruses. J. Cell Biol. 36: 231–243.

    Article  Google Scholar 

  9. Cornell, R. 1969. Cell-substrate adhesion during cell culture. An ultrastructural study. Exp. Cell Res. 58: 289–295.

    Article  PubMed  CAS  Google Scholar 

  10. Leighton, J., S. Mansukhani, and L. W. Estes. 1971. Decalcified egg shell membranes, a supporting substrate for electron microscopic cross-section of monolayers of epithelial cell line MDCK. In Vitro 6: 251–252.

    PubMed  CAS  Google Scholar 

  11. Kruse, P. F., Jr., L. N. Keen, and W. L. Whittle. 1970. Some distinctive characteristics of high density perfusion culture of diverse cell types. In Vitro 6: 75–88.

    Article  PubMed  Google Scholar 

  12. Eguchi, G., and T. S. Okada 1971. Ultrastructure of the differentiated cell colony derived from a singly isolated chondrocyte inin vitro culture. Dev. Growth Differ. (Nagoya) 12: 297–312.

    Article  CAS  Google Scholar 

  13. Guillouzo, A., P. Lenoir, and M. Bourel. 1971. Une technique d'inclusionin situ de cultures cellulaires poussant sur polystyrene pour la microscopie électronique. Rev. Eur. Etud. Clin. Biol. 16: 721–724.

    Google Scholar 

  14. Brinkley B. R., P. Murphy, and L. C. Richardson. 1967. Procedure for embedding in situ selected cells cultured in vitro. J. Cell Biol. 35: 279–283.

    Article  PubMed  CAS  Google Scholar 

  15. Anderson, W. A., and R. A. Ellis. 1965. Ultrastructure ofTrypanosoma lewisi: flagellum, microtubules, and kinctoplast. P. Protozool. 12: 483–499.

    Google Scholar 

  16. Reynolds, E. S. 1965. The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell. Biol. 17: 208–213.

    Article  Google Scholar 

  17. Brunk, U., J. L. E. Ericsson, J. Ponten, and B. Westermark. 1971. Specialization of cell surfaces in contact-inhibited human glialike cells in vitro. Exp. Cell Res. 67: 407–415.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. W. Alton Jones Cell Science Center, 12946, Lake Placid, New York

    William H. J. Douglas & John E. Elser

Authors
  1. William H. J. Douglas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John E. Elser
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Douglas, W.H.J., Elser, J.E. A method for in situ embedding of cultured cells grown on plastic surfaces. In Vitro 8, 26–29 (1972). https://doi.org/10.1007/BF02617940

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02617940

Keywords

Search

Navigation