Skip to main content
SpringerLink
Log in

Ribosomal ribonucleic acids of cultured cells: A preliminary survey of differences among mammalian species detectable by polyacrylamide gel electrophoresis

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

Ribosomal ribonucleic acids (rRNA's) of cultured cells from various species were compared by polyacrylamide gel electrophoresis. Electrophoretic mobility of the 28 S RNA component varied according to species. Human cell 28 S rRNA was distinguishable from that of monkey cells. The mobility of chimpanzee 28 S rRNA was identical to that from human cells, but different from that of monkey cells. Cat, goat, and swine cell 28 S rRNA's were distinguishable from one another and from both human and monkey cell rRNA's. The mobilities of bovine, rabbit, rat, hamster, and mouse cell 28 S rRNA's were virtually identical to that from monkey cells. No apparent correlation existed between the mobility of 28 S rRNA and the currently accepted phylogenetic position of the donors. Normal cells, tumor cells, and virus-infected cells from the same species did not differ, but in some instances embryonic and adult cells were distinguishable. The 18 S rRNA components did not show variations comparable to those of the 28 S rRNA components.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Loening, U. E. 1968. Molecular weights of ribosomal RNA in relation to evolution. J. Mol. Biol. 38: 355–365.

    Article  PubMed  CAS  Google Scholar 

  2. Loening, U. E. 1970. The mechanism of synthesis of ribosomal RNA. Symp. Soc. Gen. Microbiol. 20: 77–106.

    CAS  Google Scholar 

  3. Schaffer, F. L., M. E. Soergel, and D. C. Straube 1971. Electrophoretic analysis of ribosomal and viral ribonucleic acids with a simple technique for slicing low-concentration polyacrylamide gels. Appl. Microbiol. 22: 538–545.

    PubMed  CAS  Google Scholar 

  4. Loening, U. E. 1969. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel acrylamide-gel electrophoresis. Biochem. J. 113: 131–138.

    PubMed  CAS  Google Scholar 

  5. Fisher, M. P., and C. W. Dingman. 1971. Role of molecular conformation in determining the electrophoretic properties of polynucleotides in agarose-acrylamide composite gels. Biochemistry 10: 1895–1899.

    Article  PubMed  CAS  Google Scholar 

  6. Staynov, D. Z., J. C. Pinder, and W. B. Gratzer. 1972. Molecular weight determination of nucleic acids by gel electrophorosis in non-aqueous solution. Nature New Biol. 235: 108–110.

    Article  PubMed  CAS  Google Scholar 

  7. Morton, H. J. 1970. A survey of commercially available tissue culture media. In Vitro 6: 89–108.

    Article  PubMed  CAS  Google Scholar 

  8. Field, A. K., and J. Fong. 1964. Mouse salivary gland virus plaque assay on a stable line of mouse embryo cells. J. Bacteriol. 87: 1238–1239.

    PubMed  CAS  Google Scholar 

  9. McAllister, R. M., W. A. Nelson-Rees, E. Y. Johnson, R. W. Rongey, and M. B. Gardner. 1971. Disseminated rhabdomyosarcomas formed in kittens by cultured human rhabdomyosarcoma cells. J. Natl. Cancer Inst. 47: 603–612.

    PubMed  CAS  Google Scholar 

  10. McClure, H. M., K. H. Belden, and W. A. Pieper. 1969. Autosomal trisomy in a chimpanzee: resemblance to Down's syndrome. Science 165: 1010–1012.

    Article  PubMed  CAS  Google Scholar 

  11. Jensen, E. M., I. Zelljadt, H. C. Chopra, and M. M. Mason. 1970. Isolation and propagation of a virus from a spontaneous mammary carcinoma of a rhesus monkey. Cancer Res. 30: 2388–2393.

    PubMed  CAS  Google Scholar 

  12. Ahmed, M., S. A. Mayyasi, H. C. Chopra, I. Zelljadt, and E. M. Jensen. 1971. Mason-Pfizer monkey virus isolated from spontaneous mammary carcinoma of a female monkey. I. Detection of virus antigens by immunodiffusion, immunofluorescent, and virus agglutination techniques. J. Natl. Cancer Inst. 46: 1325–1334.

    PubMed  CAS  Google Scholar 

  13. Nelson-Rees, W. A., L. E. Hooser, and A. J. Hackett. 1972. Chronic poliovirus infection of co-cultivated monkey cells (CMMT) harboring the Mason-Pfizer monkey virus (M-PMV). J. Natl. Cancer Inst. In press.

  14. Sykes, J. A., J. Whitescarver, and L. Briggs. 1968. Observations on a cell line producing mammary tumor virus. J. Natl. Cancer Inst. 41: 1315–1327.

    PubMed  CAS  Google Scholar 

  15. Hsu, T. C., and K. Benirschke. 1967.An Atlas of Mammalian Chromosomes. Vol. 1. Springer-Verlag, New York.

    Google Scholar 

  16. Rothfels, K. H. and L. Siminovitch. 1958. An air drying technique for flattening chromosomes in mammalian cells grownin vitro. Stain Technol. 33: 73–77.

    PubMed  CAS  Google Scholar 

  17. Markov, G. G., I. Bradvarova, A. Mintcheva, P. Petrov, N. Shishkov, and R. G. Tsanev. 1969. Mycoplasma contamination of cell cultures. Interference with32P-labelling pattern of RNA. Exp. Cell Res. 57: 374–384.

    Article  PubMed  CAS  Google Scholar 

  18. Hackett, A. J. 1964. A possible morphologic basis for the autointerference phenomenon in vesicular stomatitis virus. Virology 24: 51–59.

    Article  PubMed  CAS  Google Scholar 

  19. Borun, T. W., M. D. Scharff, and E. Robbins. 1967. Preparation of mammalian polyribo-somes with the detergent Nonidet P-40. Biochim. Biophys. Acta 149: 302–304.

    PubMed  CAS  Google Scholar 

  20. Schaffer, F. L., and M. E. Soergel. 1972. Molecular weight estimates of vesicular stomatitis virus ribonucleic acids from virions, defective particles, and infected cells. Manuscript in preparation.

  21. Herrick, P. R., G. W. Baumann, D. J. Merchant, M. C. Shearer, C. Shipman, Jr., and R. G. Brackett. 1970. Serologic and karyologic evidence of incorrect identity of an animal cell line (guinea pig spleen). In Vitro 6: 143–147.

    Article  PubMed  CAS  Google Scholar 

  22. Montes De Oca, F., M. L. Macy, and J. E. Shannon. 1969. Isoenzyme characterization of animal cell cultures. Proc. Soc. Exp. Biol. Med. 132: 462–469.

    PubMed  CAS  Google Scholar 

  23. Fogh, J., N. B. Holmgren, and P. P. Ludovici. 1971. A review of cell culture contaminations. In Vitro 7: 26–41.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Naval Biomedical Research Laboratory, School of Public health, University of California, 94720, Berkeley, California

    M. E. Soergel & F. L. Schaffer

Authors
  1. M. E. Soergel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. L. Schaffer
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by the Office of Naval Research and the Bureau of Medicine and Surgery, United States Navy, under a contract between the Office of Naval Research and the Regents of the University of California.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Soergel, M.E., Schaffer, F.L. Ribosomal ribonucleic acids of cultured cells: A preliminary survey of differences among mammalian species detectable by polyacrylamide gel electrophoresis. In Vitro 8, 13–18 (1972). https://doi.org/10.1007/BF02617938

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02617938

Keywords

Search

Navigation