Summary
Dense cultures of mouse fibroblast cell line 3T6 were prepared for light microscopic study using either one of two physical substrates, glass slides or a matrix of collagencoated cellulose sponge. Whether propagated on glass or in matrix, cultures receiving a supplement of 250 μg per ml of ascorbic acid formed reticular fibers in less than 10 days. Reticular fibers were more abundant in the matrix cultures than on glass. Fibrous collagen was not found with light or electron microscopy in cultures fed on the control medium, i.e. without the ascorbic acid supplement.
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Supported by Research Grant P-442 from the American Cancer Society. Presented at the 19th annual meeting of the Tissue Culture Association in June 1968 at San Juan, Puerto Rico.
During this investigation Dr. Coldblatt was a Faculty Research Associate of the American Cancer Society, Inc. (Grant PRA-60)
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Leighton, J., Estes, L.W., Goldblatt, P.J. et al. The formation of histotypic fibrous collagen in matrix tissue culture by 3T6 mouse fibroblasts: A response to ascorbic acid. In Vitro 6, 153–161 (1970). https://doi.org/10.1007/BF02617758
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DOI: https://doi.org/10.1007/BF02617758