Summary
The growth of L cells in suspension cultures as determined by cell count and protein measurement is markedly reduced by the addition of dibutyryl cyclic adenosine 3′,5′-monophosphate (AMP) and theophylline to the culture flasks. Following the transfer of these cells to normal media the effect of dibutyryl cyclic AMP on cell growth lasts between 24 and 48 hr, and afterwards the cells have normal growth Growth arrested L cells remain agglutinable by wheat germ agglutinin.
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This investigation was supported in part by United States Public Health Service grants CA-12660, HL-13713, RR 0540210, and RR 0540211.
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Oler, A., Iannaccone, P.M. & Gordon, G.B. Suppression of growth of L cells in suspension culture by dibutyryl adenosine 3′,5′-monophosphate. In Vitro 9, 35–38 (1973). https://doi.org/10.1007/BF02615987
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DOI: https://doi.org/10.1007/BF02615987