Summary
Short ragweed (Ambrosia elatior L.) leaf cells were grown as callus and suspension cultures. Immunodiffusion tests against anti-pollen crude extract and anti-antigen E sera did not detect antigen E in the tissue cultures. However, two allergenically active fractions were isolated from the tissue cultures by ammonium sulfate precipitation, diethylaminoethyl-cellulose and Sephadex G-100 chromatography. Two fractions isolated (Sephadex G-100, fractions II and III) were electrophoretically homogeneous, carried net charges similar to that of antigen E in polyacrylamide disc electrophoresis at pH 8.9 and had molecular weights of 10,000 to 15,000 and 30,000 to 40,000, respectively. These fractions were highly skin reactive in individuals sensitive to short ragweed pollen.
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This paper was abstracted from a thesis submitted by A. Shafiee to the University of Minnesota in partial fulfillment of the Doctor of Philosophy degree requirements.
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Shafiee, A., Staba, E.J. Allergens from short ragweed leaf tissue cultures. In Vitro 9, 19–23 (1973). https://doi.org/10.1007/BF02615984
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DOI: https://doi.org/10.1007/BF02615984