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Adenosine 3′,5′-cyclic monophosphate and adrenal tumor cell morphology

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Summary

Cultured adrenal tumor cells respond to adrenocorticotropin (ACTH), adenosine 3′,5′-cyclic monophosphate (cAMP), cholera enterotoxin (CT), andEscherichia coli enterotoxin with increased steroidogenesis and a reversible alteration of monolayer morphology. The morphological response is not due specifically to the interaction of ACTH or CT with a membrane receptor site, because both cAMP and theophylline, which work intracellularly, also stimulated steroidogenesis and rounding of adrenal cells. The morphological alteration appears to be unrelated to stimulation of steroido-genesis or protein synthesis, inasmuch as CT in the presence of aminoglutethimide or cycloheximide still caused rounding and cAMP accumulation without increased steroid production. These results suggest that increased intracellular cAMP concentration may be related to the observed change of flattened adrenal cells to a spherical shape in monolayer cultures.

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Author notes

  1. Catherine N. Kwan

    Present address: Department of Bacteriology, University of California, 90024, Los Angeles, Calif

Authors and Affiliations

  1. Departments of Medicine and Microbiology, University of California, 92668, Irvine, California

    Catherine N. Kwan &  Rodney M. Wishnow

  2. Medical Research Programs (151), Veterans Administration Hospital, 90801, Long Beach, Calif

    Catherine N. Kwan &  Rodney M. Wishnow

Authors
  1. Catherine N. Kwan
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  2. Rodney M. Wishnow
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Additional information

This work was supported in part by Cancer Research Funds of the University of California and by the Medical Research Programs Veterans Administration Hospital, Long Beach, Calif.

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Kwan, C.N., Wishnow, R.M. Adenosine 3′,5′-cyclic monophosphate and adrenal tumor cell morphology. In Vitro 10, 268–273 (1974). https://doi.org/10.1007/BF02615307

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