Calcified Tissue International

, Volume 48, Issue 5, pp 326–334

Partial characterization of rat marrow stromal cells

Authors

  • David J. Simmons
    • Division Orthopedic SurgeryUniversity of Texas Medical Branch Department of Surgery
  • Patricia Seitz
    • University of Texas Medical Branch Department of Pharmacology, and Toxicology
  • Louis Kidder
    • Department of AnthropologyWashington University
  • Gordon L. Klein
    • University of Texas Medical Branch Department of Pediatrics
  • Mark Waeltz
    • Division Orthopedic SurgeryUniversity of Texas Medical Branch Department of Surgery
  • Caren M. Gundberg
    • Department of Orthopedic SurgeryYale University School of Medicine
  • Chikage Tabuchi
    • Division Bone and Mineral MetabolismWashington University School of Medicine, Jewish Hospital
  • Chyunyu Yang
    • Division Orthopedic SurgeryUniversity of Texas Medical Branch Department of Surgery
  • Ren Wen Zhang
    • Division Orthopedic SurgeryUniversity of Texas Medical Branch Department of Surgery
Laboratory Investigations

DOI: 10.1007/BF02556152

Cite this article as:
Simmons, D.J., Seitz, P., Kidder, L. et al. Calcif Tissue Int (1991) 48: 326. doi:10.1007/BF02556152

Summary

Fibroblast-like rat marrow stromal cell (CFU-F) cultures have been characterized in terms of their responsiveness to calciotropic hormones, metal ions, the nonsteroidal antiinflammatory drug, and by their putative paracrine role in the maintenance of active populations of osteoblasts at the marrow-bone interface. These studies indicate that CFU-Fs lack a complete osteoblast signature. Subconfluent CFU-Fs grown in the presence or absence of 10−7 M dexamethasone lack receptors for PTH and calcitonin, and fail to show enhanced cAMP or cGMP responses to 10−7 M 1–34 PTH (rat), or any evidence of osteocalcin production [±10−9 M 1,25-(OH)2D3]. Low concentrations of fluoride [10−12 and 10−9 M] stimulated CFU-F grownin vitro in serum-free media, though higher levels (10−7 M) did not alter normal growth patterns, indicating an action on bone cells more differentiated than CFU-Fs. Serum-free conditioned medium (CM) from control and ovariectomized (OVX)/OVX+ dihydrotachysterol-Rx rat CFU-F cultures was mitogenic for neonatal rat calvarial osteoblastsin vitro, but not for ROS 17/2.8 cells. The studies affirm the mesenchymal-like character of CFU-Fs and project their significant role in sustaining functional endosteal osteogenic cell populations.

Key words

Stromal cell Osteoblast Parathyroid hormone Calcitonin Calcitriol Osteoblast Fluoride Aluminum Osteocalcin

Copyright information

© Springer-Verlag New York Inc. 1991