Laboratory Investigations

Calcified Tissue International

, Volume 48, Issue 5, pp 326-334

Partial characterization of rat marrow stromal cells

  • David J. SimmonsAffiliated withDivision Orthopedic Surgery, University of Texas Medical Branch Department of Surgery
  • , Patricia SeitzAffiliated withUniversity of Texas Medical Branch Department of Pharmacology, and Toxicology
  • , Louis KidderAffiliated withDepartment of Anthropology, Washington University
  • , Gordon L. KleinAffiliated withUniversity of Texas Medical Branch Department of Pediatrics
  • , Mark WaeltzAffiliated withDivision Orthopedic Surgery, University of Texas Medical Branch Department of Surgery
  • , Caren M. GundbergAffiliated withDepartment of Orthopedic Surgery, Yale University School of Medicine
  • , Chikage TabuchiAffiliated withDivision Bone and Mineral Metabolism, Washington University School of Medicine, Jewish Hospital
  • , Chyunyu YangAffiliated withDivision Orthopedic Surgery, University of Texas Medical Branch Department of Surgery
  • , Ren Wen ZhangAffiliated withDivision Orthopedic Surgery, University of Texas Medical Branch Department of Surgery

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Summary

Fibroblast-like rat marrow stromal cell (CFU-F) cultures have been characterized in terms of their responsiveness to calciotropic hormones, metal ions, the nonsteroidal antiinflammatory drug, and by their putative paracrine role in the maintenance of active populations of osteoblasts at the marrow-bone interface. These studies indicate that CFU-Fs lack a complete osteoblast signature. Subconfluent CFU-Fs grown in the presence or absence of 10−7 M dexamethasone lack receptors for PTH and calcitonin, and fail to show enhanced cAMP or cGMP responses to 10−7 M 1–34 PTH (rat), or any evidence of osteocalcin production [±10−9 M 1,25-(OH)2D3]. Low concentrations of fluoride [10−12 and 10−9 M] stimulated CFU-F grownin vitro in serum-free media, though higher levels (10−7 M) did not alter normal growth patterns, indicating an action on bone cells more differentiated than CFU-Fs. Serum-free conditioned medium (CM) from control and ovariectomized (OVX)/OVX+ dihydrotachysterol-Rx rat CFU-F cultures was mitogenic for neonatal rat calvarial osteoblastsin vitro, but not for ROS 17/2.8 cells. The studies affirm the mesenchymal-like character of CFU-Fs and project their significant role in sustaining functional endosteal osteogenic cell populations.

Key words

Stromal cell Osteoblast Parathyroid hormone Calcitonin Calcitriol Osteoblast Fluoride Aluminum Osteocalcin