Abstract
A selective gas-chromatographic method is proposed for the determination of cholesterol in egg samples. The method consists of extraction of the lipid fraction and cold saponification prior to determination on a glass capillary column. The absence of cholesterol oxidation during the process was checked, and the conditions in which oxidation is avoided are discussed. The method also showed good precision (coefficient of variation=1.39%). Applying this method to different fresh and frozen egg samples, we obtained a mean content of 392 mg of cholesterol/100 g of edible portion. These values are clearly lower than the majority of results published for eggs.
Similar content being viewed by others
References
World Heath Organization,Prevention of Coronary Heart Disease, World Health Organization Technical Report Series, 678, World Health Organization, Geneve, 1982.
National Institute of Health,J.A.M.A. 253:2080 (1985).
European Atherosclerosis Society,Eur. Heart J. 8:77 (1987).
López, S.H., W.A. Krehl, R.E. Hodges and E.L. Good,Am. J. Clin. Nutr. 19:361 (1966).
Masironi, F.,Bull. WHO 42:103 (1970).
Stamler, J., R. Stamler and R.B. Shekelle, inIschemic Heart Disease, edited by J.H. de Haas, H.C. Hemker and H.A. Snellen, Leiden University Press, Leiden, 1970, p. 84.
Armstrong, B.K., J.I. Mann, A.M. Adelstein and F. Eskin,J. Chron. Dis. 28:455 (1975).
Keys, A., A. Menotti, M.J. Karvonen and C. Aravanis,Am. J. Epidemiol. 124:903 (1986).
Pyörälä, K.,Am. J. Clin. Nutr. 45:1176 (1987).
McNamara, D.J.,Current Opinion Lipidology 1:18 (1990).
Ulbricht, T.L.V., and D.A.T. Southgate,Lancet 338:985 (1991).
Kestin, M., P.M. Clifton, I.L. Rouse and P.J. Nestel,Am. J. Clin. Nutr. 50:528 (1989).
Edington, J.D., M. Geekie and R. Carter,Br. Med. J. 294:333 (1987).
Schonfeld, G., W. Patsch, L.L. Ridel, C. Nelson, M. Epstein and R.E. Olson,J. Clin. Invest. 69:1072 (1982).
Berner, Y., M. Bar-El, B. Habot and M. Shinitzky, inQuality of Poultry Products II. Eggs and Egg Products, edited by A. Oosterwoud, and A.W. de Vries, Proceedings of the 4th European Symposium on the Quality of Eggs and Egg Products, Spelderholt Center for Poultry Research and Information Services, Beekbergen, Holanda 1991, p. 91.
Organization for Economic Cooperation and Development,Food Consumption Statistics 1976–1985, Publications Service of Organizations for Economic Cooperation and Development, Paris, 1988.
Richardson, D.I.S.,Int. Eng Market Rev. 49:1, 26 (1992).
Beyer, R.S., and L.S. Jensen,J. Agr. Food Chem. 37:917 (1989).
Ulberth, F., and H. Reich,Food Chem. 43:387 (1992).
Marshall, M.W., B.A. Clevidence, R.H. Thompson and J.T. Judd,J. Food Comp. Anal. 2:2 (1989).
Folch, J., M. Lees and G.H. Sloane-Stanley,J. Biol. Chem. 226:497 (1957).
Bascoul, J., N. Domergue, M. Olle and A. Crastes de Paulet,Lipids 21:383 (1986).
Maerker, G., and J. Unruh,J. Am. Oil Chem. Soc. 63:767 (1986).
Naber, E.C., and M.D. Bigger,Poult. Sci. 64:341 (1985).
Pie, J.E., K. Spahis and C. Seillan,J. Agric. Food Chem. 38:973 (1990).
Pie, J.E., and C. Seillan,Lipids 27:270 (1992).
Thompson, R.H., and G.V. Merola,JAOAC 76:1057 (1993).
Littmann-Nienstedt, S.,Deutsche Lebensmittel-Rundschau 89:283 (1993).
Park, S.W., and P.B. Addis,Anal. Biochem. 149:245 (1985).
Bohac, C.E., K.S. Rhee, H.R. Cross and K. Ono,J. Food Sci. 53:1642 (1988).
Knoll, J.E.,J. Chromatogr. Sci. 23:422 (1985).
Scherz, H., and F. Senser,Souci, Fachman And Kraut Food Composition and Nutrition Tables, Wissenschaftliche Verlagsgesellschaft GmbH, Stuttgart, 1989, pp. 124–126.
Author information
Authors and Affiliations
About this article
Cite this article
Guardiola, F., Codony, R., Rafecas, M. et al. Selective gas chromatographic determination of cholesterol in eggs. J Am Oil Chem Soc 71, 867–871 (1994). https://doi.org/10.1007/BF02540464
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02540464