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Lymphatic absorption of oxidized cholesterol in rats

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Lipids

Abstract

The absorption of cholesterol and of cholesterol oxidation products (oxidized cholesterols) was compared in lymph-cannulated rats. We found that the lymphatic absorption of an intragastrically administered, emulsified lipid meal containing 25 mg of cholesterol or 25 mg of oxidized cholesterols, within 24 h, was approximately 67 and 30%, respectively. The absorption rate of individual oxidized cholesterols differed considerably and was approximately 30% for 7α-hydroxycholesterol, 42% for 7β-hydroxycholesterol, 32% for 5β-epoxycholesterol, 28% for 5α-epoxycholesterol, 15% for cholestanetriol and 12% for 7-ketocholesterol. Moreover, cholesterol oxidation products delayed the absorption of oleic acid as triolein. Approximately 35 and 48% of cholesterol was recovered in chylomicrons (CM) and very low density lipoprotein (VLDL), respectively. In contrast, 54 and 40% of the oxidized cholesterols was recovered in CM and VLDL, respectively, although there was a significant difference in the distribution of individual oxidized cholesterols. The results of the present study indicate that oxidized cholesterols are absorbed to a lesser extent than is cholesterol, that they disturb fat absorption and that they distribute differently between lymphatic lipoproteins.

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Abbreviations

ACAT:

acyl coenzyme A:cholesterol acyltransferase

CM:

chylomicron

EDTA:

ethylenediaminetetraacetic acid, disodium salt

GC:

gas chromatography

HDL:

high density lipoprotein

LDL:

low density lipoprotein

VLDL:

very low density lipoprotein

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Authors and Affiliations

  1. Laboratory of Nutrition Chemistry, Kyushu University School of Agriculture 46-09, Hakozaki, 812, Fukuoka, Japan

    Kyoichi Osada & Eiji Sasaki

  2. Laboratory of Food Science, Department of Food Science and Technology, Faculty of Agriculture 46-09, Kyushu University, Hakozaki 6-10-1, Higashi-ku, 812, Fukuoka, Japan

    Michihiro Sugano

Authors
  1. Kyoichi Osada
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  2. Eiji Sasaki
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  3. Michihiro Sugano
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Osada, K., Sasaki, E. & Sugano, M. Lymphatic absorption of oxidized cholesterol in rats. Lipids 29, 555–559 (1994). https://doi.org/10.1007/BF02536627

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  • DOI: https://doi.org/10.1007/BF02536627

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