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Dietary fish oil inhibits human breast carcinoma growth: A function of increased lipid peroxidation

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Lipids

Abstract

Female athymic nude mice were implanted subcutaneously with human breast carcinoma MDA-MB231. Seven to ten days later, the mice were divided into groups and fed a purified diet containing the following types of fat (% of diet):(i) 20% corn oil (CO); (ii) 15% CO:5% fish (menhaden) oil (FO); (iii) 10% CO:10% FO; (iv) 5% CO:15% FO; (v) 1% CO:19% FO; and (vi) 1% CO:19% FO plus antioxidants (α-tocopherol acetate, 2000 IU/kg diet and tertiary butyl-hydroquinone, 2% of the total fat). The linoleic acid levels (% of diet) of the groups were 12.0, 9.1, 6.2, 3.3, 0.9 and 0.9%, respectively. After 6–8 wk, the carcinomas were assessed for tumor volume (cm3) and assayed for thiobarbituric acid reactive substances (TBARS). Human breast carcinoma growth was suppressed in mice consuming FO diets without antioxidants as compared to mice fed CO; the greater the amount of dietary FO fed, the greater the carcinoma growth suppression (P<0.05). The addition of antioxidants to the FO diet significantly (P<0.05) reversed the FO-induced carcinoma growth suppression. Concentrations of TBARS in the human breast carcinomas were increased in all the FO (without antioxidants) fed mice, compared to mice fed CO; the level of increase in TBARS was directly related to the increase in the level of FO fed (P<0.05). The addition of antioxidants to the FO diet significantly (P<0.05) reduced the concentration of TBARS in the breast carcinomas. Thus, these results provide evidence that dietary FO can significantly suppress growth of human breast carcinoma MDA-MB231, even in the presence of substantial amounts of linoleic acid (3.3–9.1%). The inhibitory effect of FO on growth of these carcinomas was associated with an increased concentration of TBARS in the tumor tissue. In conclusion, dietary FO induced suppression of human breast carcinoma growth is a function, at least in part, of an accumulation of lipid peroxidation products in the tumor tissues.

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Abbreviations

CO:

corn oil

DHA:

docosahexaenoic acid

EPA:

thiobarbituric acid reactive substances

TBHQ:

tertiary butylhydroquinone

References

  1. Welsch, C.W. (1992)Cancer Res. 52, 2040s-2048s.

    PubMed  CAS  Google Scholar 

  2. Karmali, R.A., Marsh, J., and Fuchs, C. (1984)J. Natl. Cancer Inst. 73, 457–561.

    PubMed  CAS  Google Scholar 

  3. Jurkowski, J.J., and Cave, Jr., W.T. (1985)J. Natl. Cancer Inst. 74, 1145–1150.

    PubMed  CAS  Google Scholar 

  4. Gabor, H., and Abraham, S. (1986)J. Natl. Cancer Inst. 76, 1223–1229.

    PubMed  CAS  Google Scholar 

  5. Fernandes, G., Frieduchs, W., and Schultz, J. (1989)Breast Cancer Res. Treat. 14, 179A.

    Google Scholar 

  6. Pritchard, G.A., Jones, D.L., and Mansel, R.E. (1989)Brit. J. Surg. 76, 1069–1073.

    PubMed  CAS  Google Scholar 

  7. Borgeson, C.E., Pardini, L., Pardini, R.S., and Reitz, C. (1989)Lipids 24, 290–295.

    Article  PubMed  CAS  Google Scholar 

  8. Gabor, H., Blank, E.W., and Ceriani, R.L. (1990)Cancer Lett. 52, 173–178.

    Article  PubMed  CAS  Google Scholar 

  9. Gonzalez, M.J., Schemmel, R.A., Gray, J.I., Dugan, Jr., L. Sheffield, L.G., and Welsch, C.W. (1991)Carcinogenesis 12, 1231–1235.

    PubMed  CAS  Google Scholar 

  10. Braden, L.M., Carroll, K.K. (1986)Lipids 21, 285–288.

    Article  PubMed  CAS  Google Scholar 

  11. Abou-El-Ela, S.H., Prasse, K.W., Carroll, R., Wade, A.E., Dharwadkar, S., and Bunce, O.R. (1988)Lipids 23, 948–959.

    Article  PubMed  CAS  Google Scholar 

  12. Ip, C., Carter, C.A., and Ip, M.M. (1985)Cancer Res. 45, 1997–2001.

    PubMed  CAS  Google Scholar 

  13. Marmer, W.N., Maxwell, R.J. (1981)Lipids 16, 365–371.

    PubMed  CAS  Google Scholar 

  14. Crackel, R.L., Gray, J.I., and Pearson, A.M. (1988)Food Chem. 28, 187–196.

    Article  CAS  Google Scholar 

  15. Buege, J.A., and Aust, S.D. (1978)Methods Enzymol. II, 302–310.

    Article  Google Scholar 

  16. Willis, E.D. (1969)Biochem. J. 113, 315–319.

    Google Scholar 

  17. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  18. Wilkinson, L. (1989)Systat: The System for Statistics, SYSTAT, Inc., Evanston.

    Google Scholar 

  19. Abou-El-Ela, S.H., Prasse, K.W., Farrell, B.L., Carroll, R., Wade, A.E., and Bunce, O.R. (1989)Cancer Res. 49, 1434–1440.

    PubMed  CAS  Google Scholar 

  20. National Research Council (1978)Nutrient Requirements for Laboratory Animals Nutrient Requirements of Domestic Animals, No. 10, p. 44, National Academy of Sciences, Washington, D.C.

    Google Scholar 

  21. Gonzalez, M.J., Gray, J.I., Schemmel, R.A., Dugan, Jr., L., and Welsch, C.W. (1992)J. Nutr. 122, 2190–2195.

    PubMed  CAS  Google Scholar 

  22. Gonzalez, M.J. (1992)Med. Hypotheses 38, 106–110.

    Article  PubMed  CAS  Google Scholar 

  23. Spector, A.A., and Yorek, M.A. (1985)J. Lipid Res. 26, 1015–1035.

    PubMed  CAS  Google Scholar 

  24. Spector, A.A., and Burns, C.P. (1987)Cancer Res. 47, 4529–4537.

    PubMed  CAS  Google Scholar 

  25. Farber, J.L., Kyle, M.E., and Coleman, J.B. (1990)Lab. Invest. 62, 670–679.

    PubMed  CAS  Google Scholar 

  26. Roubal, W.T., and Tappel, A.L. (1966)Arch. Biochem. Biophys. 113, 5–8.

    Article  PubMed  CAS  Google Scholar 

  27. Reiss, U., and Tappel, A.L. (1973)Lipids 8, 199–202.

    PubMed  CAS  Google Scholar 

  28. Dianzani, M.U. (1982)Free Radicals, Lipid Peroxidation and Cancer (McBrien, D.C., and Slater, T.F., eds.) pp. 129–158, Academic Press, London.

    Google Scholar 

  29. Bull, A.W., Nigro, N.D., and Marnett, J.J. (1988)Cancer Res. 48, 1771–1776.

    PubMed  CAS  Google Scholar 

  30. Glasgow, W.C., Afshari, C.A., Barrett, J.C., and Eling, T.E. (1992)J. Biol. Chem. 267, 10771–10779.

    PubMed  CAS  Google Scholar 

  31. Wattenberg, L.W., Loub, W.D., Lam, L.K., and Speier, J.L. (1976)Fed. Proc. 35, 1327–1331.

    PubMed  CAS  Google Scholar 

  32. Hirose, M., Masuda, A., Inoue, T., Fukushima, S., and Ito, N. (1986)Carcinogenesis 7, 1155–1159.

    PubMed  CAS  Google Scholar 

  33. McCormick, D.L., Major, N., and Moon, R.C. (1984)Cancer Res. 44, 2858–2863.

    PubMed  CAS  Google Scholar 

  34. Ip, C. (1982)Carcinogenesis 3, 1453–1456.

    PubMed  CAS  Google Scholar 

  35. King, M.M., and McCay, P.B. (1983)Cancer Res. 43, 2485s-2490s.

    PubMed  CAS  Google Scholar 

  36. Beth, M., Berger, M.R., Aksoy, M., and Schmahl, D. (1987)Br. J. Cancer 56, 445–449.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Food Science and Human Nutrition, Michigan State University, 48824, East Lansing, Michigan

    Michael J. Gonzalez, Rachel A. Schemmel, LeRoy Dugan Jr. & J. Ian Gray

  2. Department of Pharmacology and Toxicology, Michigan State University, 357 Giltner Hall, 48824, East Lansing, MI

    Clifford W. Welsch

Authors
  1. Michael J. Gonzalez
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  2. Rachel A. Schemmel
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  3. LeRoy Dugan Jr.
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  4. J. Ian Gray
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  5. Clifford W. Welsch
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Gonzalez, M.J., Schemmel, R.A., Dugan, L. et al. Dietary fish oil inhibits human breast carcinoma growth: A function of increased lipid peroxidation. Lipids 28, 827–832 (1993). https://doi.org/10.1007/BF02536237

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  • DOI: https://doi.org/10.1007/BF02536237

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