Skip to main content
SpringerLink
Log in

Simultaneous determination ofSonchus arvensis L. triterpenes by gas chromatography-mass spectrometry

  • Methods
  • Published:
Lipids

Abstract

Sonchus arvensis L. or sow thistle has proved an excellent source of pentacylic triterpenes; triterpenes account for about 6% of the crude lipid extract or 0.2% of the dried plant. Composition of the triterpenoid fraction, as indicated by gas chromatography of the corresponding acetates, was α-amyrin (9%), β-amyrin (21%), lupeol (13%), taraxasterol (24%) and pseudo-taraxasterol (12%). A single, long-chain alcohol (16%) was also present. The nature of the triterpenoids was defined using gas chromatography and gas chromatography-mass spectrometry (GC/MS) and confirmed by direct comparison with authentic samples. The use of combined GC/MS proved extremely valuable in the simultaneous analysis of a mixture of triterpene acetates and greatly reduced the time needed to phytochemically evaluate these compounds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Chandler, R.F., Freeman, L., and Hopper, S.N. (1979) J. Ethnopharm. 1, 49–68.

    Article  CAS  Google Scholar 

  2. Behari, M., Gupta, R., Itoh, T., and Matsumoto, T. (1980) Yukagaku 29, 82–85; Chem. Abstr. 92, 211784w.

    CAS  Google Scholar 

  3. Chandler, R.F., and Hooper, S.N. (1982) Lipids, in press.

  4. Burrows, S., and Simpson, J.C.E. (1938) J. Chem. Soc. 2042–2047.

  5. Chandler, R.F., Hooper, S.N., Hooper, D.L., Jamieson, W.D., Flinn, C.G. and Safe, L.M., J. Pharm. Sci., in press.

  6. Bombardelli, E., Gabetta, B., Martinelli, E.M., and Mustichi, G. (1979) Fitoterapia 50, 11–24.

    CAS  Google Scholar 

  7. Method Ca. 6b-53 (1972) The Official and Tentative Methods of the American Oil Chemists' Society, 3rd edn., American Oil Chemists' Society, Champaign, IL.

  8. Safe, L.M., Wong, C.J., and Chandler, R.F. (1974) J. Pharm. Sci. 63, 464–466.

    Article  PubMed  CAS  Google Scholar 

  9. Hooper, S.N., and Chandler, R.F. (1978) J. Pharm. Sci. 67, 1157–1159.

    Article  PubMed  CAS  Google Scholar 

  10. Madrigal, R.V., Plattner, R.D., and Smith, C.R. Jr. (1975) Lipids 10, 208–213.

    Article  CAS  Google Scholar 

  11. Hartwell, J.L., and Abbott, B.J. (1969) Adv. Pharm. Chemother. 7, 117–209.

    Article  CAS  Google Scholar 

  12. Hartwell, J.L. (1976) Cancer Treatment Rep. 60, 1031–1067.

    CAS  Google Scholar 

  13. Miles, D.H., Kokpol, U., Zalkow, L.H., Steindel, S.J., and Nabors, J.B. (1974). J. Pharm. Sci. 63, 613–615.

    Article  PubMed  CAS  Google Scholar 

  14. Pettit, G.R. (1977) Biosynthetic Products for Cancer Chemotherapy, Vol. 1, pp. 61–96, 177–186, Plenum Press, New York, NY.

    Google Scholar 

  15. Pettit, G.R., and Cragg, G.M. (1978) Biosynthetic Products for Cancer Chemotherapy, Vol. 2, pp. 11–60, 121–126, Plenum Press, New York, NY.

    Google Scholar 

  16. Pettit, G.R., and Ode, R.H. (1979) Biosynthetic Products for Cancer Chemotherapy, Vol. 3, pp. 9–18, 65–124, Plenum Press, New York, NY.

    Google Scholar 

  17. Chandler, R.F., Hooper, S.N., and Ismail, H.A. (1979) Can. J. Pharm. Sci. 14, 61–62.

    CAS  Google Scholar 

  18. Subbiah, M.T.R. (1973) Am. J. Clin. Nutr. 26, 219–225.

    PubMed  CAS  Google Scholar 

  19. Chaturvedi, A.K., Parmar, S.S., Nigam, S.K., Bhatnagar, S.C., Misra, G., and Sastry, B.V.R. (1976) Pharmacol. Res. Commun. 8, 199–210.

    Article  PubMed  CAS  Google Scholar 

  20. Farnsworth, N.R., and Cordell, G.A. (1976) Lloydia 39, 420–455.

    PubMed  CAS  Google Scholar 

  21. Benoit, P.S., Fong, H.H.S., Svoboda, G.H., and Farnsworth, N.R. (1976) Lloydia 39, 160–171.

    PubMed  CAS  Google Scholar 

  22. Shibata, S. (1977) in New Natural Products and Plant Drugs with Pharmacological, Biological or Therapeutic Activity (Wagner, H., and Wolff, P., eds.) pp. 177–196, Springer-Verlag, New York, NY.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Pharmacy, Dalhousie University, Halifax, Nova Scotia, Canada

    S. N. Hooper & R. F. Chandler

  2. Atlantic Research Laboratory, National Research Council, Halifax, Nova Scotia, Canada

    E. Lewis & W. D. Jamieson

Authors
  1. S. N. Hooper
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. F. Chandler
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Lewis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. W. D. Jamieson
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Hooper, S.N., Chandler, R.F., Lewis, E. et al. Simultaneous determination ofSonchus arvensis L. triterpenes by gas chromatography-mass spectrometry. Lipids 17, 60–63 (1982). https://doi.org/10.1007/BF02535123

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02535123

Keywords

Search

Navigation