Abstract
Effects of dietary n−6 and n−3 fatty acids (FAs) on blood pressure (BP) and tissue phospholipid (PL) FA composition in spontaneously hypertensive (SHR) and normotensive Wistar-Kyoto (WKY) rats were compared. Male weanling SHR and WKY were fed a fat-free semisynthetic diet supplemented with 10% (w/w) fats containing (a) 78% 18∶2n−6 (LA-rich), (b) 20% LA and 55% 18∶3n−3 (LN-rich), or (c) 11% LA and 3% LN (CON) for seven weeks. Dietary fats did not affect the BP elevation, but significantly altered the FA composition of brain, adrenal gland, renal medulla and cortex PL in SHR. The LA-rich diet increased n−6 FA while it reduced n−3 FA levels. The levels of 20∶4n−6 were not significantly different between animals fed the LA-rich and the CON diets. LN-rich diet increased the levels of n−3 FAs, while it reduced those of n−6 FAs. However, the extent of change was significantly less in SHR than in WKY. In all dietary groups, SHR, as compared to WKY, had a relatively higher level of the 2 series prostaglandin (PG) precursor, 20∶4n−6, and a relatively lower level of the 1 and 3 series PG precursors, 20∶3n−6 and 20∶5n−3. The possibility that the unbalanced eicosanoid FA precursor levels might contribute to the development of hypertension in this animal model is discussed.
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Abbreviations
- AA:
-
arachidonic acid
- ANOVA:
-
analysis of variance
- BF3-methanol:
-
boron trifluoride methanol
- BP:
-
blood pressure
- CON:
-
control oil
- DGLA:
-
dihomo-gamma-linolenic acid
- DHA:
-
docosahexaenoic acid
- EPA:
-
eicosapentaenoic acid
- FAs:
-
fatty acids
- HCO:
-
hydrogenated coconut oil
- LA:
-
linoleic acid
- LN:
-
linolenic acid
- LSO:
-
linseed oil
- MUFAs:
-
monounsaturated fatty acids
- PG:
-
prostaglandin
- PGI1 :
-
prostacyclin
- PGI3 :
-
prostacyclin
- PL:
-
phospholipids
- PUFAs:
-
polyunsaturated fatty acids
- SD:
-
standard deviation
- SFAs:
-
saturated fatty acids
- SFO:
-
safflower oil
- SHR:
-
spontaneously hypertensive rats
- TLC:
-
thinlayer chromatography
- TX:
-
thromboxane
- TXA3 :
-
inactive thromboxane
- WKY:
-
normotensive Wistar-Kyoto rats
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Watanabe, Y., Huang, YS., Simmons, V.A. et al. The effect of dietary n−6 and n−3 polyunsaturated fatty acids on blood pressure and tissue fatty acid composition in spontaneously hypertensive rats. Lipids 24, 638–644 (1989). https://doi.org/10.1007/BF02535081
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DOI: https://doi.org/10.1007/BF02535081