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Molecular species analysis of phosphoglycerides from the ripe roes of cod (Gadus morhua)

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Lipids

Abstract

Molecular species of the 3,5-dinitrobenzoyl derivatives of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) were quantitated by UV detection at 254 nm after reversed-phase HPLC using solvent systems modified from Takamuraet al. (Lipids 21, 356–361, 1986). Three isocratic solvent systems were used and a total of 39 different molecular species detected. Four species, 16∶0/20∶5, 18∶1/20∶5, 16∶0/22∶6 and 18∶1/22∶6 contributed 67.2% and 61.8% of PC and PE respectively but only 23.0% of PI. In PI the most important species was 18∶0/20∶4 at 36.7% but this species only constituted 0.7% in each of PC and PE. Small amounts of dipolyunsaturated species were also found in PC and PE.

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Abbreviations

PUFA:

polyunsaturated fatty acid

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PS:

phosphatidylserine

PI:

phosphatidylinositol

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Authors and Affiliations

  1. NERC Unit of Aquatic Biochemistry, School of Molecular and Biological Sciences, University of Stirling, FK9 4LA, Stirling, Scotland

    M. V. Bell

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  1. M. V. Bell
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Additional information

Molecular species are abbreviated as follows: e.g., 16∶0/20∶5 PC is 1-palmitoyl-2-eicosapentaenoyl-sn-glycero-3-phosphocholine.

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Bell, M.V. Molecular species analysis of phosphoglycerides from the ripe roes of cod (Gadus morhua). Lipids 24, 585–588 (1989). https://doi.org/10.1007/BF02535073

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  • DOI: https://doi.org/10.1007/BF02535073

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