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The effect of hydroxyethyl starch and other plasma volume substitutes on endothelial cell activation; an in vitro study

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Abstract

Objective

To study the effect of medium molecular weight hydroxyethyl starches on endothelial cell and neutrophil activation in vitro.f

Setting

Laboratory analysis.

Methods

The effects of albumin and hydroxyethyl starch on the neutrophil adhesion molecule (CD11bCD18), with and without liposaccharide stimulation, were studied in whole blood. E-selection expression on human umbilical vein endothelial cells was stimulated with lipopolysaccharide alone and in the presence of either albumin or hydroxyethyl starch. The effect of albumin and hydroxyethyl starches on rapid endothelial cell activation was studied using von Willebrand factor release as a marker.

Measurements and results

Hydroxyethyl starches but not albumin inhibited stimulated vWF release in a dose dependent manner. No effect was seen on endothelial Eselectin or neutrophil CD11bCD18 expression.

Conclusions

These results suggest a possible beneficial role of hydroxyethyl starches in the inhibition of endothelial activation thus preventing neutrophil adhesion during sepsis syndrome.

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References

  1. Bone RC (1991) The pathogenesis of sepsis. Ann Intern Med 115:457–469

    PubMed  CAS  Google Scholar 

  2. Knaus WA, Draper EA, Wagner DP, Zimmerman JE (1985) Prognosis in acute organ-system failure. Ann Surg 202:685–693

    PubMed  CAS  Google Scholar 

  3. Tighe D, Moss RF, Boghossian S, Heath MF, Chessum B, Bennett ED (1989) Multi-organ damage resulting from experimental faecal peritonitis. Clin Sci 76:269–276

    PubMed  CAS  Google Scholar 

  4. Hattori R, Hamilton KK, McEver RP, Sims PJ (1989) Complement proteins C5b-9 induce secretion of high molecular weight multimers of endothelial von Willebrand factor and translocation of granule membrane protein GMP-140 to the cell surface. J Biol Chem 264:9053–9060

    PubMed  CAS  Google Scholar 

  5. Hattori R, Hamilton KK, Fugates RD, McEver RP, Sims PJ (1989) Stimulated secretion of endothelial von Willebrand factor is accompanied by rapid redistribution to the cell surface of the intracellular granule membrane protein GMP-140. J Biol Chem 264:7768–7771

    PubMed  CAS  Google Scholar 

  6. Lawrence MB, Springer TA (1991) Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins. Cell 60:859–873

    Article  Google Scholar 

  7. Mulligan MS, Polley MJ, Bayer RJ, Nunn MF, Paulson JC, Ward PA (1992) Neutrophil-dependent acute lung injury. Requirement for P-selectin (GMP-140). J Clin Invest 90:1600–1607

    PubMed  CAS  Google Scholar 

  8. McEver RP, Beckstead JH, Moore KL, Marshall-Carlson L, Bainton DF (1989) GMP-140, a platelet alpha granule membrane protein, is also synthesized by vascular endothelial cells and is localized in Weibel-Palade bodies. J Clin Invest 84:92–99

    PubMed  CAS  Google Scholar 

  9. Bevilacqua MP, Pober JS, Mendrick DL, Cotran RS, Gimbrone MA (1987) Identification of an inducible endothelial-leukocyte adhesion molecule. Proc Natl Acad Sci 84:9238–9242

    Article  PubMed  CAS  Google Scholar 

  10. Lawrence MB, Springer TA (1991) Leukocyte roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins. Cell 60:859–873

    Article  Google Scholar 

  11. Hakkert BC, Kuijpers TW, Leeuwenberg JF, van Mourik JA, Roos D (1991) Neutrophil and monocyte adherence to and migration across monolayers of cytokine-activated endothelial cells: the contribution of CD18, ELAM-1 and VLA-4. Blood 78:2721–2726

    PubMed  CAS  Google Scholar 

  12. Webb AR, Righe D, Moss RF, Al-Saady NA, Hynd JW, Bennett ED (1991) Advantages of narrow-range, medium molecular weight hydroxyethyl starch for volume maintenance in a porcine model of faecal peritonitis. Crit Care Med 19:409–416

    PubMed  CAS  Google Scholar 

  13. Webb AR, Moss RF, Tighe D, Al-Saady NA, Mythen MG, Joseph AE, Bennett ED (1992) A narrow Range, medium molecular weight pentastarch reduces structural organ damage in a hyperdynamic porcine model of sepsis. Intensive Care Med 18:348–355

    Article  PubMed  CAS  Google Scholar 

  14. Jaffe EA, Nachman RL, Becker CG, Minick CR (1973) Culture of human endothelial cells derived from umbilical veins. J Clin Invest 52:2745–2756

    Article  PubMed  CAS  Google Scholar 

  15. Dubravec DB, Spriggs DR, Rodrick ML (1990) Circulating human peripheral blood granulocytes synthesis and secrete tumour necrosis factor α. Proc Natl Acad Sci USA 87:6758–6761

    Article  PubMed  CAS  Google Scholar 

  16. Prober JS, Gimbrone MA, Lapierre LA, Mendrick DL, Fiers W, Rothlein R, Springer TA (1986) Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor and immune interferon. J Immunol 137:1893–1896

    Google Scholar 

  17. Sica A, Matsushima K, van Damme J, Wang JM, Polentarutti N, Dejana E, Colotta F, Mantovani A (1990) II-1 transcriptionally activates the neutrophil chemotactic factor/IL-8 gene in endothelial cells. Immunology 69:548–553

    PubMed  CAS  Google Scholar 

  18. Broudy VC, Kaushansky K, Segal GM, Harlan JM, Adamson JW (1986) Tumor necrosis factor type α stimulates human endothelial cells to produce granulocyte/macrophage colony-stimulating factor. Proc Natl Acad Sci USA 83:7467–7471

    Article  PubMed  CAS  Google Scholar 

  19. Yuo A, Kitagawa S, Kasahara T, Matsushima K, Saito M, Takaku F (1991) Stimulation and priming of human neutrophils by interleukin-8: cooperation with tumour necrosis factor and colony-stimulating factors. Blood 78:2708–2714

    PubMed  CAS  Google Scholar 

  20. Meyrick B, Ryan US, Brigham KL (1986) Direct effects of E. coli endotoxin on structure and permeability of pulmonary endothelial monolayers and the endothelial layers of intimal explants. Am J Pathol 122:140–151

    PubMed  CAS  Google Scholar 

  21. Rubin DB, Matthay MA, Weinberg PF, Murray JF (1985) Factor VIII antigen: possible plasma marker of progressive acute lung injury in patients with sepsis. Am Rev Respir Dis 131:A142

    Google Scholar 

  22. Zikria BA, King TC, Stanford J, Freeman HP (1989) A biophysical approach to capillary permeability. Surgery 105:625–631

    PubMed  CAS  Google Scholar 

  23. Lockwood DNJ, Bullen C, Machin SJ (1988) A severe coagulopathy following volume replacement with hydroxyethyl starch in a Jehovahs Witness. Anaesthesia 43:391–393

    PubMed  CAS  Google Scholar 

  24. Macintyre E, Mackie IJ, Ho D, Tinker J, Bullen C, Machin SJ (1985) The haemostatic effects of hydroxyethyl Starch (HES) used as a Volume expander. Intensive Care Med 11:300–303

    Article  PubMed  CAS  Google Scholar 

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Collis, R.E., Collins, P.W., Gutteridge, C.N. et al. The effect of hydroxyethyl starch and other plasma volume substitutes on endothelial cell activation; an in vitro study. Intensive Care Med 20, 37–41 (1994). https://doi.org/10.1007/BF02425053

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  • DOI: https://doi.org/10.1007/BF02425053

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