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Quantitative bone histology in 84 normal American subjects

Micromorphometric analysis and evaluation of variance in iliac bone

  • Clinical Investigations
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Summary

Quantitative bone histology was done in undecalcified sections of iliac crest bone specimens obtained from 84 normal American individuals. Samples were obtained within 12 h after death in a vertical and horizontal manner from both the right and left iliac crests. In addition to the determination of normal values of micromorphometric parameters of bone in these healthy American subjects, the following studies were carried out: (a) comparison of variance of micromorphometric parameters of bone obtained from the right versus left iliac bone (40 pairs), (b) comparison of micromorphometric parameters of bone obtained in a vertical versus horizontal manner (12 pairs), (c) evaluation of variance with increasing distance from the compact zone in bone samples obtained in a vertical manner (44 pairs), (d) analysis of variation between bone samples obtained more anteriorly versus posteriorly along the iliac crest (N=40), (e) comparison of differences in micromorphometric parameters obtained from age-matched men versus premenopausal women (N=12), and (f) plotting of histograms for assessment of distribution of micromorphometric parameters. The results show that histomorphometric data of bone cannot be easily compared when different techniques are employed for obtaining bone samples. Sampling variations are kept smaller when bone specimens are obtained in a vertical manner. Anterior/posterior variation does not cause major sampling error. If ranges of variation are taken into account, quantitative bone histology is a valuable tool for assessment of bone structure and bone cells.

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References

  1. Malluche HH, Sherman D, Meyer W, Manaka R, Massry SG (1982) A new semiautomatic method for quantitative static and dynamic bone histology. Calcif Tissue Int 34:439–448

    CAS  PubMed  Google Scholar 

  2. Manaka R, Malluche HH (1981) A program package for quantitative analysis of histologic structure and remodelling dynamics of bone. Comput Programs Biomed (in press)

  3. Malluche HH, Ritz E, Hodgson M, Kutschera H, Krause G, Seiffert U, Gati A, Lange HP (1975) Skeletal lesions and calcium metabolism in early renal failure. In Moorhead, Mion, Baillod (eds): Dialysis, Transplantation, Nephrology. Pitman Medical, pp 443–450

  4. Burck HC (1973) In: Histologische Technik. G. Thieme Verlag, Stuttgart, pp 110–113

    Google Scholar 

  5. Ritz E, Malluche HH, Krempien B, Mehls O (1977) Bone histology in renal insufficiency. In David DS (ed): Calcium Metabolism in Renal Failure and Nephrolithiasis. John Wiley & Sons, New York, pp 197–233

    Google Scholar 

  6. Dixon WJ, Brown MB (1977) Biomedical Computer Programs. P. Series, University of California Press, Los Angeles p 149

    Google Scholar 

  7. Schenk P (1973) Standard values (histomorphometry) iliac crest cancellous bone. In Jaworski ZFG (ed): Proceedings of the First Workshop on Bone Morphometry. University of Ottawa, Ottawa, pp 392–394

    Google Scholar 

  8. Schulz A, Delling G (1973) Histomorphometric preparation and technique determination of trabecular bone volume. In Jaworski ZFG (ed): Proceedings of the First Workshop on Bone Morphometry. University of Ottawa, Ottawa, pp 106–108

    Google Scholar 

  9. Courpron P, Meunier P, Bressot C, Giroux JM (1976) Amount of bone in iliac crest biopsy. Significance of the trabecular bone volume. Its values in normal and in pathological conditions. In Meunier PJ (ed): Proceedings of Second International Workshop. Lyon, France, pp 39–53

  10. Bordier PJ, Tun CS (1972) Quantitative histology of metabolic bone disease. Clin Endocrinol Metab 1:197–215

    Article  Google Scholar 

  11. Melsen F, Melsen B, Mosekilde L, Bergman S (1978) Histomorphometric analysis of normal bone from the iliac crest. Acta Pathol Microbiol Scand 86:70–81

    CAS  Google Scholar 

  12. Hruska KA, Teitelbaum SL, Kopelman R, Richardson CA, Miller P, Debman J, Martin K, Slatolsky E (1978) The predictability of the histological features of uremic bone disease by non-invasive techniques. Metab Bone Dis Rel Res 1:39–44

    Article  Google Scholar 

  13. Sherrard DJ, Baylink DJ, Wergedal JE, Maloney NA (1974) Quantitative histological studies on the pathogenesis of uremic bone disease. J Clin Endocrinol Metab 39:119–35

    Article  CAS  PubMed  Google Scholar 

  14. Whitehouse WJ (1977) Cancellous bone in the anterior part of the iliac crest. Calcif Tissue Res 23:67–76

    Article  CAS  PubMed  Google Scholar 

  15. Xipell JM, Brown DJ (1979) Histology of normal bone—a computerized study in the iliac crest. Pathology 11:235–240

    CAS  PubMed  Google Scholar 

  16. Melsen F, Melsen B, Mosekilde L (1978) An evaluation of the quantitative parameters applied in bone histology. Acta Pathol Microbiol Scand 86:63–69

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Division of Nephrology and Department of Medicine, University of Southern California School of Medicine, 90033, Los Angeles, California, USA

    Hartmut H. Malluche, Wolfgang Meyer, David Sherman & Shaul G. Massry

Authors
  1. Hartmut H. Malluche
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  2. Wolfgang Meyer
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  3. David Sherman
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  4. Shaul G. Massry
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Malluche, H.H., Meyer, W., Sherman, D. et al. Quantitative bone histology in 84 normal American subjects. Calcif Tissue Int 34, 449–455 (1982). https://doi.org/10.1007/BF02411283

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  • DOI: https://doi.org/10.1007/BF02411283

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