Skip to main content
SpringerLink
Log in

Adorption of human fibrinogen and human serum albumin onto polyethylene

  • Published:
Annals of Biomedical Engineering Aims and scope Submit manuscript

Abstract

The adsorption of human fibrinogen (HFb) onto polyethylene at pH 7.4 and 37°C is irreversible with the saturated value 0.51 μg/cm2. The established dependence of the concentration of irreversibly adsorbed HFb on the concentration of HFb in solution does not reflect an equilibrium between adsorption and desorption and cannot be described by an adsorption isotherm. No reversible adsorption of HFb and HSA at 37°C has been observed. At lower temperatures, both reversibly and irreversibly adsorbed HFb have been detected.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Blombäck, B. and M. Blombäck. Purification of bovine and human fibrinogen.Acta Chem. Scand. 10:147–159, 1956.

    Google Scholar 

  2. Brash, J. L. and D. J. Lyman. Adsorption of plasma proteins in solution to uncharged hydrohobic polymer surfaces.J. Biomed. Mater. Res. 3:175–189, 1969.

    Article  CAS  PubMed  Google Scholar 

  3. Brynda, E., J. Drobník, J. Vacík, and J. Kálal. Protein sorption on surfaces measured by fluorescence labels.J. Biomed. Mater. Res. 12:55–65, 1978.

    Article  CAS  PubMed  Google Scholar 

  4. Brynda, E., M. Houska, Z. Pokorná, N. A. Cepalova, Yu. V. Moiseev, and J. Kálal. Irreversible adsorption of human albumin onto polyethylene film.J. Bioeng. 2:411–418, 1978.

    CAS  PubMed  Google Scholar 

  5. Dillman, W. J., Jr., and J. F. Miller. On the adsorption of serum protein on polymer membrane surfaces.J. Colloid Interface Sci. 44:221–241, 1973.

    Article  CAS  Google Scholar 

  6. Lee, R. G., C. Adamson, and S. W. Kim. Competitive adsorption of plasma proteins onto polymer surfaces.Thromb. Res. 4:485–490, 1974.

    Article  CAS  PubMed  Google Scholar 

  7. Morrissey, B. W. The adsorption and conformation of plasma proteins: a physical approach.Ann. N.Y. Acad. Sci. 283:50–64, 1977.

    CAS  Google Scholar 

  8. Vroman, L., A. L. Adams, M. Klings, G. C. Fischer, P. C. Munoz, and R. P. Solensky. Reactions of formed elements of blood with plasma proteins at interfaces.Ann. N.Y. Acad. Sci. 283: 65–76, 1977.

    CAS  Google Scholar 

  9. Vroman, L. and E. F. Leonard.The Behavior of Blood and Its Components at Interfaces. New York: Annals of the New York Academy of Sciences, 1977.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Macromolecular Chemistry, Czechoslovak Academy of Sciences, Heyrovského Sq. 2, 162 06, Prague 6, Czechoslovakia

    E. Brynda, M. Houska & J. Kálal

  2. Institute of Chemical Physics, Academy of Sciences of the U.S.S.R., Moscow, USSR

    N. A. Cepalova

Authors
  1. E. Brynda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Houska
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Kálal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. A. Cepalova
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brynda, E., Houska, M., Kálal, J. et al. Adorption of human fibrinogen and human serum albumin onto polyethylene. Ann Biomed Eng 8, 245–252 (1980). https://doi.org/10.1007/BF02364480

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02364480

Keywords

Search

Navigation