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The effect of storage temperature on reducing sugar concentration and the activities of three amylolytic enzymes in tubers of the cultivated potato,Solanum tuberosum L.

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Summary

Reducing sugar content, and activities of three starch hydrolysing enzymes, alpha-amylase, beta-amylase and debranching enzyme were measured over several months in tubers of five cultivars stored at 4°C or 10°C. Cultivars differed in their sensitivity to storage temperature. Reducing sugar content of tubers and the activities of three starch hydrolysing enzymes increased sharply during the first weeks of storage at 4°C. At 10°C, reducing sugar content, and the activity of the three enzymes remained constant or increased only slightly.

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References

  • Bailey, K. M., I. D. J. Phillips & D. Pitt, 1978. The role of buds and gibberellin in dormancy and the mobilisation of reserve materials in potato tubers.Annals of Botany 42: 649–657.

    CAS  Google Scholar 

  • Bielinska-Czarnecka, M. & K. Bialek, 1977. Amylase and acid phosphatase activity in potato tubers treated with gibberellic acid and stored at 2°C and 8°C.Acta Agrobotanica 30: 95–101.

    CAS  Google Scholar 

  • Brown, J., G. R. Mackay, H. Bain, D. W. Griffith & M. J. Allison, 1990. The processing potential of tubers of cultivated potato,Solanum tuberosum L., after storage at low temperatures. 2. Sugar concentration.Potato Research 33: 219–227.

    Google Scholar 

  • Bruinsma, J., 1962. A survey of recent Japanese research on dormancy of potato tubers.European Potato Journal 5: 195–203.

    Google Scholar 

  • Burton, W. J., 1982. Post harvest physiology of food crops. Longman, London, 331 pp.

    Google Scholar 

  • Cochrane, M. P., C. M. Duffus, M. J. Allison & G. R. Mackay, 1991a. Amylolytic activity in stored potato tubers. I. Estimation using p-nitrophenyloligosaccharides.Potato Research 34: 325–332.

    CAS  Google Scholar 

  • Cochrane, M. P., C. M. Duffus, M. J. Allison & G. R. Mackay, 1991b. Amylolytic activity in stored potato tubers. II. The effect of low temperature storage on the activities of alpha- and beta-amylase and alpha-glucosidase in potato tubers.Potato Research 34: 333–341.

    CAS  Google Scholar 

  • Davies, H. V. & H. A. Ross, 1987. Hydrolytic and phosphorolytic enzyme activity and reserve mobilisation in sprouting tubers of potato (Solanum tuberosum L.).Journal of Plant Physiology 126: 387–397.

    CAS  Google Scholar 

  • Ewing, E. E., 1974. Potato chip colour.American Potato Journal 51: 242–243.

    Google Scholar 

  • Hesen, J. C., 1983. The reducing content of potatoes.IBVL Publication 353b 12 pp.

  • Isherwood, F. A., 1973. Effects of changes in storage temperature on the metabolism of the potato tuber.Proceedings of the 5th Triennial Conference of the EAPR Norwich, pp. 156–157.

  • McCleary, B. V. & R. Codd, 1989. Measurement of beta-amylase in cereal flours and commercial enzyme preparationsJournal of Cereal Science 9: 17–34.

    CAS  Google Scholar 

  • McCleary, B. V. & H. Sheehan, 1987. Measurement of cereal alpha-amylase: A new assay procedure.Journal of Cereal Science 6: 237–251.

    CAS  Google Scholar 

  • Mackay, G. R., J. Brown & C. J. W. Torrance, 1990. The processing potential of tubers of the cultivated potato,Solanum tuberosum L., after storage at low temperatures. 1. Fry colour.Potato Research 33: 211–218.

    Google Scholar 

  • Morrell, S. & T. ap Rees, 1986. Sugar metabolism in developing tubers ofSolanum tuberosum.Phytochemistry 25: 1579–1585.

    CAS  Google Scholar 

  • Nowak, J., 1977. Biochemical changes in stored potato tubers with different rest periods. II. Influence of the storage temperature and isopropyl phenylcarbamates on enzyme activities.Zeitschrift für Pflanzenphysiologie 81: 125–140.

    CAS  Google Scholar 

  • Pollock, C. & T. ap Rees, 1975. Activities of enzymes of sugar metabolism in cold stored tubers ofSolanum tuberosum L.Phytochemistry 14: 613–617.

    CAS  Google Scholar 

  • Samotus, B. & M. Leja, 1973. The extraction of amylolytic enzymes from potato tuber tissue and the determination of their activity.Ziemniak (1973): 21–29.

    Google Scholar 

  • Sowokinos, J. R., E. C. Lulai & J. A. Knoper, 1985. Translucent tissue defects inSolanum tuberosum L. I. Alterations in amyloplast membrane integrity, enzyme activities, sugars and starch content.Plant Physiology 78: 489–494.

    CAS  Google Scholar 

  • Sowokinos, J. R., P. H. Orr, J. A. Knoper & J. L. Varns, 1987. Influence of potato storage and handling stress on sugars, chip quality and integrity of the starch (Amyloplast) membrane.American Potato Journal 64: 213–226.

    Google Scholar 

  • Talburt, W. F. & O. Smith, 1967. Potato processing. Avi Publishing Company, Connecticut.

    Google Scholar 

  • Talley, E. A. & W. L. Porter, 1970. Chemical composition of potatoes VII. Relationship of the free amino acid concentrations to specific gravity and storage time.American Potato Journal 47: 214–224.

    CAS  Google Scholar 

  • Weaver, M. L. & H. Timm, 1983. Significance of the fructose/glucose ratio in screening potato breeding lines with processing potential.American Potato Journal 60: 329–338.

    CAS  Google Scholar 

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Cottrell, J.E., Duffus, C.M., Paterson, L. et al. The effect of storage temperature on reducing sugar concentration and the activities of three amylolytic enzymes in tubers of the cultivated potato,Solanum tuberosum L.. Potato Res 36, 107–117 (1993). https://doi.org/10.1007/BF02358725

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