Summary
An automated sample preparation module, (the automated sample preparation with extraction columns, ASPEC), was interfaced with a capillary gas chromatograph (GC) by means of an on-column interface. The system was optimised for the determination of the antidepressant trazodone in plasma. The clean-up of untreated plasma was carried out with disposable C18 cartridges, using several washing steps. The analyte was desorbed with methanol, the extract was diluted on-line with buffer and preconcentrated on a PLRP-S trapping column. The trapping column was dried by purging with nitrogen gas. Desorption (phase switching) of the trapping column was carried out with ethyl acetate which was introduced into a retention gap using partially concurrent solvent evaporation conditions. Trazodone was determined by GC with flame ionisation detection (FID). With a 1 ml sample, this resulted in a detection limit for trazodone in plasma of 3 ng/ml. The system showed good linearity and repeatability in the range 0.01–1 μg/ml, thus covering the range of pharmacokinetic/dynamic-to-therapeutic concentrations of trazodone in plasma.
Preliminary results for benzodiazepines are promising. They indicate that the use of a selective detector such as the nitrogen/phosphorus detector, is to be recommended.
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References
J. J. Vreuls, G. J. de Jong, R. T. Ghijsen, U. A. Th. Brinkman, J. Ass. Off. Anal. Chem.77, 306 (1994).
J. J. Vreuls, A. J. H. Louter, U. A. Th. Brinkman in H.-J. Stan, Ed., “Chemistry of Plant Protection”, Springer, Berlin, 1995, in press.
V. Gianesello, L. Bolzani, E. Brenn, A. Gazzaniga, HRC & CC11, 99 (1988).
V. Gianesello, E. Brenn, G. Figini, A. Gazzaniga, J. Chromatogr.473, 343 (1989).
F. Munari, K. Grob, HRC & CC11, 172 (1988).
R. E. Gammans, W. W. Bullen, R. R. Covington, J. W. Russel, J. Chromatogr.339, 303 (1985).
N. Rifal, C. B. Levtzow, C. M. Howlett, J. C. Philips, N. C. Parker, R. E. Cross, J. Anal. Toxicol.12, 150 (1988).
O. Andriollo, C. Lartique-Mattei, J. L. Chabard, H. Bargnoux, J. Petit, J. A. Berger, J. F. Pognat, J. Chromatogr.575, 301 (1992).
A. Scotto di Tella, C. di Nunzio, P. Ricci, G. Parisi, J. Anal. Toxicol.10, 233 (1986).
R. N. Gupta, M. Lew, J. Chromatogr.342, 442 (1985).
R. Caldwell, R. J. Flanagan, J. Chromatogr.345, 187 (1985).
G. R. van de Hoff, S. M. Gort, R. A. Baumann, P. van Zoonen, U. A. Th. Brinkman, HRC & CC14, 465 (1991).
G. R. van der Hoff, R. A. Baumann, U. A. Th. Brinkman, P. van Zoonen, J. Chromatogr.644, 367 (1993).
R. Said, D. Robinet, C. Barbier, J. Sartre, C. Huquet, J. Chromatogr.5302, 11 (1990).
J. J. Vreuls, V. P. Goudriaan, G. J. de Jong, U. A. Th. Brinkman, HRC14, 475 (1991).
J. J. Vreuls, G. J. de Jong, R. T. Ghijsen, U. A. Th. Brinkman, J. Chromatogr.625, 237 (1992).
P. J. M. Kwakman, J. J. Vreuls, R. T. Ghijsen, U. A. Th. Brinkman, Chromatographia34, 41 (1992).
A. J. H. Louter, U. A. Th. Brinkman, R. T. Ghijsen, J. Microcol. Sep.5, 303, (1993).
Y. Gaillard, J.-P. Gay-Montchamp, M. Ollagnier, J. Chromatogr.622, 197 (1993).
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Louter, A.J.H., van der Wagt, R.A.C.A. & Brinkman, U.A.T. On-line automated sample preparation-capillary gas chromatography for the analysis of plasma samples. Chromatographia 40, 500–506 (1995). https://doi.org/10.1007/BF02290259
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DOI: https://doi.org/10.1007/BF02290259