Originals

Chromatographia

, Volume 17, Issue 1, pp 9-15

Investigation of selectivity in reversed-phase liquid chromatography-effects of stationary and mobile phases on retention of homologous series

  • H. ColinAffiliated withLaboratoire de Chimie Analytique Physique, Ecole Polytechnique
  • , A. M. KrstulovićAffiliated withLaboratoire de Chimie Analytique Physique, Ecole Polytechnique
  • , M. -F. GonnordAffiliated withLaboratoire de Chimie Analytique Physique, Ecole Polytechnique
  • , G. GuiochonAffiliated withLaboratoire de Chimie Analytique Physique, Ecole Polytechnique
  • , Z. YunAffiliated withDepartment of Chemistry, University of Peking
  • , P. JanderaAffiliated withDepartment of Analytical Chemistry, University of Chemical Technology

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Summary

Extrapolation of log k'vs. nc plots for homologous series chromatographed with different mobile phases leads to a common intersection point. Its coordinates depend on the series and the solvent used, and can be rationalized on the basis of the retention model of interaction indices.

The existence of this convergence point is reflected in the linear relationship of logα-logβ, which permits the optimization of chromatographic separations in the region of solvent compositions where these two parameters can be varied independently.

Key Words

Reversed-Phase Liquid Chromatography Selectivity Interaction Indices Homologous Series Optimization