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Characterization ofCampylobacter jejuni lipopolysaccharide

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Abstract

Lipopolysaccharides were isolated from dehydratedCampylobacter jejuni by combination of the phenol-chloroform-petroleum ether and phenol-water extraction techniques. Biochemical characterizations of lipopolysaccharide were performed on the two fractions of highest purity. Neutral sugar analyses detected galactose, glucose, trace amounts of mannose, and an unidentified deoxy-hexose. The primary amino sugars were galactosamine, glucosamine, and glucosamine-phosphate. Chemical analyses of other lipopolysaccharide components included phosphate, 3-deoxy-d-manno-octulosonic acid (KDO), and fatty acids. The predominant fatty acids were 3-hydroxytetradecanoic and hexadecanoic acids with lesser amounts of tetradecanoic acid. 3-Hydroxytetradecanoic acids were bound to lipid A by both amide and ester linkages.

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Authors and Affiliations

  1. Department of Biochemistry and Microbiology, College of Osteopathic Medicine, Oklahoma State University, 1111 West 17th Street, 74107, Tulsa, OK, USA

    Robert S. Conrad

  2. Max-Planck-Institute for Immunobiology, Freiburg, Germany

    Chris Galanos

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  1. Robert S. Conrad
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  2. Chris Galanos
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Conrad, R.S., Galanos, C. Characterization ofCampylobacter jejuni lipopolysaccharide. Current Microbiology 21, 377–379 (1990). https://doi.org/10.1007/BF02199441

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