Abstract
Mitochondrial DNA polymorphisms in 15 specimens of three species of slow lorises-Nycticebus coucang, N. intermedius, andN. pygmaeus-were analyzed in order to study the evolutionary relationships among the species. Eight restriction types were observed in the samples. Phylogenetic trees constructed on the basis of genetic distances showed that the slow lorises sort into two clusters: four types ofN. coucang and three types ofN. intermedius plus one type ofN. pygmaeus. Our results suggest that there are two valid species in the genusNycticebus-N. coucang, andN. pygmaeus-and thatN. intermedius should be included withinN. pygmaeus. Divergence between the two species may have begun 2.7 Ma (million years ago). Evolution of gross morphology, chromosomes, and mitochondrial DNA in the slow lorises appears to be concordant.
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Avise, J. C., Arnold, J., Ball, R. M., Bermingham, E., Lamb, T., Neigel, J. E., Reeb, C. A. and Saunders N. C. (1987). Intraspecific phylogeography: The mitochondrial DNA bridge between population genetics and systematics.Annu. Rev. Ecol. Syst. 18: 489–522.
Brown, G. G., and Simpson, M. V. (1981). Intra- and interspecific variation of the mitochondrial genome inRattus norvegicus andRattus rattus: Restriction enzyme analysis of variant mitochondrial DNA molecules and their evolutionary relationships.Genetics 97: 125–143.
Brown, W. M. (1980). Polymorphism in mitochondrial DNA of human as revealed by restriction endonuclease analysis.Proc. Natl. Acad. Sci. USA 77: 3605–3609.
Brown, W. M. (1983). Evolution of animal mitochondrial DNA. In Nei, M., and Koehn, R. K. (eds.),Evolution of Genes and Proteins, Sinauer, Sunderland, Mass., pp. 62–88.
Brown, W. M., George, M., Jr., and Wilson, A. C. (1979). Rapid evolution of animal mitochon-drial DNA.Proc. Natl. Acad. Sci. USA 76: 1967–1971.
Brown, W. M., Prager, E. M., Wang, A., and Wilson, A. C. (1982). Mitochondrial DNA sequences of primates: Tempo and mode of evolution.J. Mol. Evol. 18: 225–239.
Corbet, G. B., and Hill, J. E. (1991).A World List of Mammalian Species, British Museum (Natural History), Oxford.
Dao, V. T. (1960). Sur un nouvelle especedNycticebus, au Vietnam.Zool. Anz. 164: 240–243.
Easteal, S. (1991). The relative rate of DNA evolution in primates.Mol. Biol. Evol. 8: 115–127.
Ferris, S. D., Brown, W. M., Davidson, W. S., and Wilson, A. C. (1981). Extensive polymorphism in the mitochondrial DNA of apes.Proc. Natl. Acad. Sci. USA 78: 6319–6323.
Ferris, S. D., Sage, R. D., Prager, E. M., Ritte, U., and Wilson, A. C. (1983). Mitochondrial DNA evolution in mice.Genetics 105: 681–721.
Groves, C. P. (1971). Systematics of the genusNycticebus.Proc. 3rd Int. Congr. Primatol., Zurich 1970, Vol. 1, Basel, Karger, pp. 44–53.
Harihara, S., Saitou, N., Hirai, M., Aoto, N., Terao, K., Cho, F., Honjo, S., and Omoto, K. (1988). Differentiation of mitochondrial DNA types inMacaca fascicularis.Primates 29: 117–127.
Hayasaka, K., Horai, S., Gojobori, T., Shotake, T., Nozawa, K., and Matsunaga, E. (1988). Phylogenetic relationships among Japanese, rhesus, Formasan, and crab-eating monkeys, inferred from restriction-enzyme analysis of mitochondrial DNAs.Mol. Biol. Evol. 5: 270–281.
Hill, W. C. O. (1953).Primates: Comparative Anatomy and Taxonomy, Vol. 1. Strepsirhini, New York, pp. 159–163.
Lansman, R. A., Shade, R. O., Shapira, J. F., and Avise, J. C. (1981). The use of restriction endonucleases to measure mitochondrial DNA sequence relatedness in natural populations, III. Techniques and potential applications,J. Mol. Evol. 17: 214–226.
Nei, M., and Li, W. H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases.Proc. Natl. Acad. Sci. USA 76: 5269–5273.
Nowak, R. M., and Paradiso, J. L. (1983).Walker's, Mammals of the World, University Press, Baltimore and London, pp. 361–363.
Petter, J. J., and Petter-Rousseaux, A. (1979). Classification of the prosimians. In Doyle, G. A., and Martin, R. D. (eds.),The Study of Prosimian Behavior, Academic Press New York, pp. 281–286.
Saitou, N., and Nei, M. (1987). The neighbour-joining method: A new method for reconstruction phylogenetic tree.Mol. Biol. Evol. 4: 406–425.
Sneath, P. H. A., and Sokal, R. R. (1973).Numerical Taxonomy: The Principles and Practice of Numerical Classification, W. H. Freeman, San Francisco.
Wilson, A. C., Cann, R. L., Carr, S. M., George, M., Jr., Gyllensten, U. B., Helm-Bychowski, K. M., Higuchi, R. G., Palumbi, S. R., Prager, E. M., Sage, R. D., and Stoneking, M. (1985). Mitochondrial DNA and two perspectives on evolutionary genetics.Biol. Linn. Soc. 26: 375–400.
Zhang, Y. P., and Shi, L. M. (1989). Mitochondrial DNA polymorphism in five species ofMacaca. Chine.J. Genet. 16: 325–338.
Zhang, Y. P., Zhang, P., and Shi, L. M. (1989). Restriction maps of mitochondrial DNA of slow loris and tree shrew.Zool. Res. 10 (Suppl): 79–89.
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Zhang, Yp., Chen, Zp. Phylogeny of the slow lorises (genusNycticebus): An approach using mitochondrial DNA restriction enzyme analysis. International Journal of Primatology 14, 167–175 (1993). https://doi.org/10.1007/BF02196510
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DOI: https://doi.org/10.1007/BF02196510