Microscopic green algae and cyanobacteria in high-frequency intermittent light
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The effects of fluctuations in the irradiance onScenedesmus quadricauda, Chlorella vulgaris andSynechococcus elongatus were studied in dilute cultures using arrays of red light emitting diodes. The growth rate and the rate of photoinhibition were compared using intermittent and equivalent continuous light regimes in small-size (30 ml) bioreactors. The CO2 dependent photosynthetic oxygen evolution rates in the intermittent and continuous light regimes were compared for different light/dark ratios and different mean irradiances. The kinetics of the electron transfer reactions were investigated using a double-modulation fluorometer. The rates of photosynthetic oxygen evolution normalized to equal mean irradiance were lower or equal in the intermittent light compared to the maximum rate found in the equivalent optimal continuous light regime. In contrast, the growth rates in the intermittent light can be higher than the growth rate in the equivalent continuous light. Photoinhibition is presented as an example of a physiological process affecting the growth rate that occurs at different rates in the intermittent and equivalent continuous lights. The difference in the dynamics of the redox state of the plastoquinone pool is proposed to be responsible for the low photoinhibition rates observed in the intermittent light.
- Barta DJ, Tibbitts TW, Bula RJ, Morrow RC (1992) Evaluation of light emitting diode characteristics for a space-based plant irradiation source. Adv. Space Res. 12: 141–149.
- Bula RJ, Morrow RC, Tibbitts TW, Ignatius RW, Martin TS, Barta DJ (1991) Light emitting diodes as a radiation source for plants. Hort. Science 26: 203–205.
- Dera J, Gordon HR (1968) Light field fluctuations in the photic zone. Limmnol. Oceanogr. 13: 697–699.
- Doucha J, Livansky K (1995) Novel outdoor thin-layer high density microalgal culture system: Productivity and operational parameters. Algological Studies 76: 129–147.
- Emerson R, Arnold W (1932) A separation of the reactions in photosynthesis by means of intermittent light. J. gen. Physiol. 15: 391–420.
- Falkowski PG (1984) Physiological responses of phytoplankton to natural light regimes J. Plankton Res. 6: 295–307.
- Gordon HR, Smith JM, Brown OB (1971) Spectra of underwater light-field fluctuations in the photic zone. Bull. mar. Sci. 21: 467–470.
- Grobbelaar JU (1994) Turbulence in mass algal cultures and the role of light/dark fluctuations. J. appl. Phycol. 6: 331–335.
- Grobbelaar JU Kroon BMA, B-Wiersma T, Mur LR (1992) Influence of medium frequency light/dark cycles of equal duration on the photosynthesis and respiration ofChlorella pyrenoidosa. Hydrobiologia 238: 53–62.
- Grobbelaar JU, Nedbal L, Tichy L, Setlik I (1995) Variation in some photosynthetic characteristics of microalgae cultured in outdoor thin-layerd sloping reactors. J. appl. Phycol. 7: 175–184.
- Kok B (1953) Experiments on photosynthesis byChlorella in flashing light. In J. S. Burlew (ed.) Algal Culture from Laboratory to Pilot Plant, pp. 63–75, Carnegie Institute, Washington, DC.
- Kroon BMA (1994) Variability of photosystem II quantum yield and related processes inChlorella pyrenoidosa (Chlorophyta) acclimated to an oscillating light regime simulating a mixed photic zone. J. Phycol. 30: 841–852.
- Laws EA, Terry KL, Wickman J, Chalup MS (1983) A simple algal production system designed to utilize the flashing light effect. Biotech. Bioengin. 25: 2319–2335.
- Legendre L, Rochet M, Demers S (1986) Sea-ice microalgae to test the hypothesis of photosynthetic adaptation to high frequency light fluctuations. J. exp. mar. Biol. Ecol. 97: 321–326.
- Meyers J (1994) The 1932 experiments. Photosynth. Res. 40: 303–310.
- Nedbal L, Trtffek M (1995) Diffuse-light dual-modulatin fluorometer: Monitoring of electron transfer reactions inSynechococcus elongatus exposed to intermittent light. In Mathis P (ed.), Photosynthesis: From Light to Biosphere, Vol. V: 813–816, Kluwer Academic Publishers.
- Phillips JN, Myers J (1954) Growth rate ofChlorella in flashing light. Plant Physiol. 29: 152–161.
- Prasil O, Adir N, Ohad I (1992) Dynamics of photosystem II: mechanisms of photoinhibition and recovery processes. In Barber J (ed.), Topics in Photosynthesis, The Photosystems: Structure, Function and Molecular Biology. Vol 11, 295–348. Elsevier, Amsterdam.
- Richmond A, Vonshak A (1978) Spirulina culture in Israel. Arch. Hydrobiol. Beih., Ergebn. Limnol. 11: 274–280.
- Sager JC, Giger W (1980) Re-evalution of published data on the relative photosynthetic efficiency of intermittent and continuous light Agricult. Meteorology 22: 289–302.
- Schenck H (1957) On the focusing of sunlight by ocean waves. J. opt. Soc. Amer. 47: 653–657.
- Šetlik I, Sust V, Malek I (1970) Dual purpose circulation unit for large scale culture of algae in temperate zones. I. Basic design considerations and scheme of pilot plant. Algological Studies 11: 111–164.
- Tennessen DJ, Bula RJ, Sharkey TD (1995) Efficiency of photosynthesis in continuous and pulsed light emitting diode irradiation. Photosynth. Res. 44: 261–269.
- Terry KL (1986) Photosynthesis in modulated light: Quantitative dependence of photosynthetic enhancement on flashing rate. Biotechnol. Bioengng. 28: 988–995.
- Tichý V, Poulson M, Grobbelaar JU, Xiong F, Nedbal L (1995) Photosynthesis, growth and photoinhibition of microalgae exposed to intermittent light. In P. Mathis (ed.), Photosynthesis: From Light to Biosphere, Vol. V: 1029–1032, Kluwer Academic Publishers.
- Walsh P, Legendre L (1983) Photosynthesis of natural phytoplankton under high frequency light fluctuations simulating those induced by sea surface waves. Limnol. Oceanogr. 28: 688–697.
- Warburg O (1919) Über die Geschwindigkeit der photochemischen Kohlenziurezetzung in lebenden Zellen. Biochem. Z. 100: 230–270.
- Weller S, Franck J (1941) Photosynthesis in flashing light. J. Phys. Chem. 45: 1359–1373.
- Whitmarsh J (1992) Mobile electron carriers in thylakoids. In: Staehelin LA and Amtzen CJ (eds). Encyclopedia of Plant Physiology. Vol. 19, pp. 508–527, Springer Verlag, Berlin.
- Microscopic green algae and cyanobacteria in high-frequency intermittent light
Journal of Applied Phycology
Volume 8, Issue 4-5 , pp 325-333
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- Kluwer Academic Publishers
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- growth rate
- intermittent light
- oxygen evolution
- Industry Sectors
- Author Affiliations
- 1. National Research Center for Photosynthesis and Global Climate Change, Institute of Microbiology AVČR, Opatovický mlýn, 37981, Třebon, Czech Republic
- 2. Department of Botany and Genetics, UOFS, Bloemfontein, 9300, South Africa