Zusammenfassung
Der Einfluß der Gewebekonzentrationen von Östradiol, Östron und Progesteron auf den Gehalt cytoplasmatischer Östradiol- und Progesteron-bindender Rezeptoren sowie der Enzymaktivität der 17β-Hydroxysteroiddehydrogenase (17β-HSD) im normalen Myometrium und Myomen während verschiedener reproduktiver Phasen der Frau wurde untersucht. Die gemessenen Parameter waren in ihren Konzentrationen von der endokrinen Situation abhängig.
Die Gewebespiegel von Östradiol und Progesteron verhielten sich im normalen Myometrium während des mensuellen Cyclus ähnlich wie die Serumkonzentrationen. Die Östronspiegel des normalen Myometrium hingegen waren — im Vergleich zum Serum — in der lutealen Phase höher als in der follikulären. Die Östradiol-bindenden Rezeptoren wiesen ihre höchsten Konzentrationen im Cytoplasma in der proliferativen Cyclushälfte auf und fielen post ovulationem stark ab. Der Gehalt an cytoplasmatischen Progesteron-Rezeptoren war mittcyclisch am höchsten. Die enzymatische Aktivität der 17β-HSD stieg in der frühen Sekretionsphase etwa um das Zehnfache und korrelierte mit der Zunahme der Östron-Gewebespiegel.
Die auffälligsten Unterschiede zwischen normalem Myometrium und Myom waren: niedrige Östron-Gewebespiegel und nur ein geringer Anstieg der 17β-HSD-Aktivität in der zweiten Cyclushälfte. Die cytoplasmatischen Rezeptorkonzentrationen für Östradiol und Progesteron waren während des Cyclus stets niedrig und zeigten keinerlei signifikante Schwankungen.
Nach der Menopause entsprachen die Konzentrationen des cytoplasmatischen Östradiolrezeptor denen der follikulären Cyclusphase. Die Progesteron bindenden Rezeptorproteine sowie die 17β-HSD-Aktivität zeigten hingegen sehr niedrige Werte. Im Myometrium von Frauen, die hormonelle Kontrazeptiva nahmen, entsprachen die cytoplasmatischen Rezeptorkonzentrationen mittcyclischen Werten. Die 17β-HSD-Aktivität war trotz niedriger Progesteron-Gewebespiegel hoch.
Summary
A study has been made on the influence of estradiol, estrone, and progesterone on cytoplasmic estradiol and progesterone receptors and the 17β-hydroxysteroid dehydrogenase (17β-HSD) activity in normal myometrium and in fibromyomata during various phases of the menstrual cycle.
The concentrations of estradiol, estrone, and progesterone in normal myometrium during the menstrual cycle were similar to those in serum except for higher levels of tissue estrone in the luteal phase. The number of estradiol-binding sites was highest during the proliferative phase and decreased after ovulation. The highest progesterone receptor content was found at midcycle. The 17β-HSD activity increased approximately 10-fold during the early secretory phase and correlated with the increase of tissue estrone levels.
In fibromyomata there were low tissue estrone concentrations and only a moderate increase of 17β-HSD activity during the secretory phase. Furthermore, the number of cytoplasmic estradiol and progesterone receptor binding sites was low and did not change during the menstrual cycle, thus indicating in fibromyomata a defect in formation of cytoplasmic receptors, especially for progesterone.
After the menopause the number of estradiol-binding sites was high, as in the proliferative phase of the cycle, whereas the number of progesterone-binding sites and the amount of 17β-HSD activity were extremely low. Myometrium from women on oral contraceptives showed an intermediate number of cytoplasmic receptors and a high 17β-HSD activity despite low tissue progesterone concentrations.
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This paper is dedicated to Prof. Dr. H. Lax on the occasion of his 70th birthday
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Eiletz, J., Genz, T., Pollow, K. et al. Sex steroid levels in serum, myometrium, and fibromyomata in correlation with cytoplasmic receptors and 17β-HSD activity in different age-groups and phases of the menstrual cycle. Arch. Gynecol. 229, 13–28 (1980). https://doi.org/10.1007/BF02109824
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DOI: https://doi.org/10.1007/BF02109824