Skip to main content
SpringerLink
Log in

Epithelial cell electrolytes in relation to transepithelial sodium transport across toad urinary bladder

  • Published:
The Journal of Membrane Biology Aims and scope Submit manuscript

Summary

Aspects of the relationships between cellular composition and transepithelial sodium transport across toad urinary bladder are reviewed. Changes in cellular sodium produced by amiloride, vasopressin, aldosterone, hypoxia, ouabain, and sodium-free media are consistent with a cellular sodium transport pool. Metabolic studies suggest that this pool gains its sodium from the mucosal medium and that there is little recycling of sodium between cell and serosal medium. One-third of the cellular potassium equilibrates readily with serosal potassium. The rate of exchange of potassium is much less than the rate of sodium transport supporting the contention that sodium transport in this tissue is electrogenic. Studies with36Cl suggest that chloride does not cross the apical cellular membranes, but exchanges with serosal chloride. Possible relationships between transepithelial sodium transport and cellular volume regulation are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bentley, P.J. 1968. Amiloride: A potent inhibitor of sodium transport across the toad bladder.J. Physiol. (London) 195:317

    Google Scholar 

  2. Canessa, M., Labarca, P., Leaf, A. 1976. Metabolic evidence that serosal sodium does not recycle through the active transepithelial transport pathway of toad bladder.J. Membrane Biol. 30:65

    Google Scholar 

  3. Ehrlich, E.N., Crabbé, J. 1968. The mechanism of action of Amipramizide.Pfluegers Arch. 302:79

    Google Scholar 

  4. Essig, A., Leaf, A. 1963. The role of potassium in active transport of sodium by the toad bladder.J. Gen. Physiol. 46:505

    Google Scholar 

  5. Frazier, H.S., Leaf, A. 1963. The electrical characteristics of active sodium transport in the toad bladder.J. Gen. Physiol. 46:491

    Google Scholar 

  6. Kleinzeller, A., Knotková, A. 1964. The effect of ouabain on the electrolyte and water transport in kidney cortex and liver slices.J. Physiol. (London) 175:172

    Google Scholar 

  7. Koefoed-Johnsen, V., Ussing, H.H. 1958. The nature of the frog skin potential.Acta Physiol. Scand. 42:298

    Google Scholar 

  8. Leaf, A. 1967. On the functional structure of the transport system in the toad bladder. Proc. 3rd Int. Congr. Nephrol. Washington 1966, Vol., pp. 18–26, Karger, Basel, New York

    Google Scholar 

  9. Leaf, A., Macknight, A.D.C. 1972. The site of the aldosterone induced stimulation of sodium transport.J. Steroid Biochem. 3:237

    Google Scholar 

  10. Macknight, A.D.C. 1968. Water and electrolyte contents of rat renal cortical slices in potassium-free media and media containing ouabain.Biochim. Biophys. Acta 150:263

    Google Scholar 

  11. Macknight, A.D.C. 1977. Contribution of mucosal chloride to chloride in toad bladder epithelial cells.J. Membrane Biol. 36:55

    Google Scholar 

  12. Macknight, A.D.C., Civan, M.M., Leaf, A. 1975. The sodium transport pool in toad urinary bladder epithelial cells.J. Membrane Biol. 20:365

    Google Scholar 

  13. Macknight, A.D.C., Civan, M.M., Leaf, A. 1975. Some effects of ouabain on cellular ions and water in epithelial cells of toad urinary bladder.J. Membrane Biol. 20:387

    Google Scholar 

  14. Macknight, A.D.C., DiBona, D.R., Leaf, A., Civan, M.M. 1971. Measurement of the composition of epithelial cells from the toad urinary bladder.J. Membrane Biol. 6:108

    Google Scholar 

  15. Macknight, A.D.C., Leaf, A. 1977. Regulation of cellular volume.Physiol. Rev. (in press)

  16. Macknight, A.D.C., Leaf, A., Civan, M.M. 1971. Effects of vasopressin on the water and ionic composition of toad bladder epithelial cells.J. Membrane Biol. 6:127

    Google Scholar 

  17. Macknight, A.D.C., McLaughlin, C.W. Transepithelial sodium transport and CO2 production by the toad urinary bladder in the absence of serosal sodium.J. Physiol. (London) (in press)

  18. McIver, D.J.L., Macknight, A.D.C. 1974. Extracellular space in some isolated tissues.J. Physiol. (London) 239:31

    Google Scholar 

  19. Nellans, H.N., Schultz, S.G. 1976. Relations among transepithelial sodium transport, potassium exchange and cell volume in rabbit ileum.J. Gen. Physiol. 68:441

    Google Scholar 

  20. Rick, R., Dörge, A., Macknight, A.D.C., Leaf, A., Thurau, K. 1978. Electron microprobe analysis of the different epithelial cells of toad urinary bladder.J. Membrane Biol. 39:257

    Google Scholar 

  21. Robinson, B.A., Macknight, A.D.C. 1976. Relationships between serosal medium potassium concentration and sodium transport in toad urinary bladder. I. Effects of different medium potassium concentrations on electrical parameters.J. Membrane Biol. 26:217

    Google Scholar 

  22. Robinson, B.A., Macknight, A.D.C. 1976. Relationships between serosal medium potassium concentration and sodium transport in toad urinary bladder. II. Effects of different medium potassium concentrations on epithelial cell composition.J. Membrane Biol. 26:239

    Google Scholar 

  23. Robinson, B.A., Macknight, A.D.C. 1976. Relationships between serosal medium potassium concentration and sodium transport in toad urinary bladder. III. Exchangeability of epithelial cellular potassium.J. Membrane Biol. 26:269

    Google Scholar 

  24. Ussing, H.H. 1949. The distinction by means of tracers between active transport and diffusion.Acta Physiol. Scand. 19:43

    Google Scholar 

  25. Ussing, H.H., Zerahn, K. 1951. Active transport of sodium as the source of electric current in the short-circuited isolated frog skin.Acta Physiol. Scand. 23:110

    Google Scholar 

  26. Whittembury, G. 1968. Sodium and water transport in kidney proximal tubular cells.J. Gen. Physiol. 51:303s.

    Google Scholar 

  27. Zerahn, K. 1969. Nature and localization of the sodium pool during active transport in the isolated frog skin.Acta Physiol. Scand. 77:272

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology, University of Otago Medical School, Dunedin, New Zealand

    Anthony D. C. Macknight & Alexander Leaf

  2. Department of Medicine, Massachusetts General Hospital and Harvard Medical School, 02114, Boston, Massachusetts

    Anthony D. C. Macknight & Alexander Leaf

Authors
  1. Anthony D. C. Macknight
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alexander Leaf
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Macknight, A.D.C., Leaf, A. Epithelial cell electrolytes in relation to transepithelial sodium transport across toad urinary bladder. J. Membrain Biol. 40 (Suppl 1), 247–260 (1978). https://doi.org/10.1007/BF02026009

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02026009

Keywords

Search

Navigation