Skip to main content
Log in

Some factors affecting the haemagglutinin of soybean

  • Originalarbeiten
  • Published:
Zeitschrift für Ernährungswissenschaft Aims and scope Submit manuscript

Summary

The water extract of unheated defatted ground soybean (soybean meal) contained haemagglutinin activity 7137 units per g soybean meal and carbohydrate content 6.34 g per 100 g soybean meal. After adjusting the pH-value of the water extract to pH 4.4, and centrifuging it, the supernatant contained most of the carbohydrate content (6.27 g per 100 g soybean meal), while noticeable decrease in the haemagglutinin was found. The DEAE-cellulose chromatography of the acetone precipitate from the water extract of soybean meal gave five protein peaks. The first four peaks showed the presence of haemagglutinin and about 40% of the total haemagglutinin were present in the first peak. The haemagglutinin activity of the water extract of soybean meal was measured at different temperatures for different intervals. The activity was easily affected by the heat treatment and it was destroyed completely after five minutes by heating at 100 ‡C and the activation energy was 9.45–10.26 kcal/mole.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Goddard, V. R., L. B. Mendel: Biol. Chem.82, 447 (1929).

    CAS  Google Scholar 

  2. Liener, I. E., E. G. Hill: J. Nutr.49, 609 (1953).

    CAS  Google Scholar 

  3. Liener, I. E.: J. Biol. Chem.233, 401 (1958).

    CAS  Google Scholar 

  4. Lis, H., N. Sharon, F. Katchalski: Biochim. Biophys. Acta83, 376 (1964).

    CAS  Google Scholar 

  5. Hintz, H. F., D. E. Hogue, L. Kroog: J. Nutr.93, 77 (1976).

    Google Scholar 

  6. A. O. A. C. Official methods of analysis of the association of official agricultural chemists. Published by the association of official agricultural chemists, Washington (1970).

    Google Scholar 

  7. Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers, F. Smith: Anal. Chem.28, 350 (1956).

    Article  CAS  Google Scholar 

  8. Markham, R.: Biochem. J.36, 790 (1942).

    Article  CAS  Google Scholar 

  9. Lowry, O. H., W. J. Rosebrough, A. Fair, R. J. Bandall: J. Biol. Chem.193, 265 (1951).

    CAS  Google Scholar 

  10. Wolf, W. J., D. A. Sly, W. F. Kwolek: Cereal Chem.43, 80 (1966).

    CAS  Google Scholar 

  11. Sambeth, W., M. C. Nesheim, J. A. Serafin: J. Nutr.92, 479 (1967).

    CAS  Google Scholar 

  12. Catsimpoolas, Nicholas, Meyer, W. Edwin: Arch. Biochem. Biophys.132, 279 (1969). Cited by Chem. Abstr.71, 35 909 (1969).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hussein, L., El-Saied, H.M. & Abu-Salem, F. Some factors affecting the haemagglutinin of soybean. Z Ernährungswiss 19, 233–237 (1980). https://doi.org/10.1007/BF02023781

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02023781

Navigation