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Histamine release by free radicals: Effect of defibrotide (DFT) and its fraction “P.o. 085 D V”

  • Histamine Release from Mast Cells and Basophils
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Abstract

The effects of defibrotide (DFT) and its fraction P.O. 085 D V on rat serosal mast cell histamine release evoked by free radicals were studied. Free radical production was obtained with: a) metabolic activation of arachidonic acid and cocaine by means of liver microsomes (S-10 mix) from polychlorinated biphenyl mixture (PCB)-treated rats; b) metabolic activation of arachidonic acid by means of prostaglandin-H-synthetase (PHS) and c) human platelet aggregation induced by arachidonic acid. DFT and its fraction reduced histamine release evoked by aggregating human platelets; P.O. 085 D V significantly reduced histamine release evoked by metabolic activation of both cocaine and arachidonic acid in the presence of S-10 mix, while DFT was less active on the cocaine-S 10 mix system and ineffective on the arachidonic acid-S 10 mix system. Both drugs were unable to decrease histamine release induced by metabolic activation of arachidonic acid in the presence of PHS.

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Authors and Affiliations

  1. Department of Preclinical and Clinical Pharmacology, University of Florence, Viale G. B. Morgagni, 65, 50134, Florence, Italy

    F. Gambassi, M. Lupini, M. G. Di Bello, L. Mugnai, E. Masini & P. F. Mannaioni

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  1. F. Gambassi
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  2. M. Lupini
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  3. M. G. Di Bello
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  4. L. Mugnai
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  5. E. Masini
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  6. P. F. Mannaioni
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Gambassi, F., Lupini, M., Di Bello, M.G. et al. Histamine release by free radicals: Effect of defibrotide (DFT) and its fraction “P.o. 085 D V”. Agents and Actions 38, C206–C208 (1993). https://doi.org/10.1007/BF01996461

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