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Formaldehyde-Schiff's reagent as a nucleolar stain

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Summary

The use of formaldehyde-Schiff's reagent as a nucleolar stain has been described. Using different digestion procedures, it was confirmed that the stain is specific for RNA. It can be suitably used as a nucleolar stain, particularly in plant materials after a short TCA extraction, which probably extracts the nonbound RNA.

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References

  1. S. Ghosh, Naturwissenschaften,61, 687 (1974).

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  2. A. G. E. Pearse,Theorctidal and Applied Histochemistry, 3rd edn. Churchill, London 1968), vol. 1, p. 647.

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Authors and Affiliations

  1. Chromosome Research Centre, Department of Botany, University of Calcutta, 700019, Calcutta, India

    S. Ghosh

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  1. S. Ghosh
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Ghosh, S. Formaldehyde-Schiff's reagent as a nucleolar stain. Experientia 32, 673–674 (1976). https://doi.org/10.1007/BF01990228

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  • DOI: https://doi.org/10.1007/BF01990228

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