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Comparison of four genotyping assays for epidemiological study of methicillin-resistantStaphylococcus aureus

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Abstract

Twenty-six methicillin-resistantStaphylococcus aureus strains were genetically differentiated by interrepeat PCR and the results compared with those of ribotyping, pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA analysis obtained in a previous study for the same strains. The comparison showed that the PCR-mediated assays were as discriminatory as PFGE, whereas ribotyping was the least powerful genotyping method. Due to the ease of performance, PCR fingerprinting may become the method of choice for establishing clonal relationships amongStaphylococcus aureus isolates.

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Authors and Affiliations

  1. University Hospital Dijkzigt, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands

    A. van Belkum & R. Bax

  2. Laboratory of Bacteriology, Louis Pasteur University, Strasbourg, France

    G. Prevost

Authors
  1. A. van Belkum
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  2. R. Bax
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  3. G. Prevost
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van Belkum, A., Bax, R. & Prevost, G. Comparison of four genotyping assays for epidemiological study of methicillin-resistantStaphylococcus aureus . Eur. J. Clin. Microbiol. Infect. Dis. 13, 420–424 (1994). https://doi.org/10.1007/BF01972002

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  • DOI: https://doi.org/10.1007/BF01972002

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