Summary
Using squid giant axon, an experimental survey was performed on restoration of the membrane excitability which had been partially suppressed. Among reagents examined, a combination of 400mm KF, 50 μm tyrosine, 1mm ATP, 1mm Mg ions and 5 μm cAMP was found to induce the restoration of the excitability to a large extent. Further addition of a small amount of either porcine brain microtubule proteins or the squid axoplasm was found to support complete restoration. The experiments suggest that tubulin-tyrosine ligase contained in the porcine brain microtubule protein fraction or the squid axoplasm maintains the coupling between cytoskeletal structures and the plasma membrane.
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Matsumoto, G., Sakai, H. Restoration of membrane excitability of squid giant axons by reagents activating tyrosine-tubulin ligase. J. Membrain Biol. 50, 15–22 (1979). https://doi.org/10.1007/BF01868785
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DOI: https://doi.org/10.1007/BF01868785