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The source and action of histamine in the isolated guinea-pig gallbladder

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Abstract

We have investigated the effects of histamine on motility of the gallbladder and characterized the receptor types involved. Histamine and the histamine H1-receptor agonist, 2-thiazolylethylamine (2-TEA) contracted the isolated guinea-pig gallbladder strip in a dose dependent manner. The contractile response to histamine was shifted to the right by the H1-receptor antagonist, mepyramine. In pre-contracted gallbladder strips, the H2-receptor agonist dimaprit reduced the tension generated in a dose dependent fashion. The histamine H2-receptor antagonist, ranitidine shifted the histamine concentration effect curve to the left and attenuated the dose dependent relaxations elicited at high concentrations. The histamine H3-receptor agonist, (R)-α-methylhistamine (RMHA) elicited dose dependent contraction of the tissue which was significantly inhibited in the presence of mepyramine. The effects of electrical field stimulation (EFS) on the strips were not significantly altered by the presence of RMHA (10−10−10−7 M) indicating little pre-synaptic H3 activity in this tissue. Histamine immunoreactivity (IR) was detected in gallbladder whole mount preparations of the mucosa and the muscularis/serosa. The histamine IR appeared cell bound in cells of varying morphological characteristics but no IR was detected in nerve fibres or cell bodies (ganglia). Alcian blue staining was consistent with the distribution of histamine IR cells as mast cells. The results indicate that histamine is distributed in the guinea-pig gallbladder and it can regulate contractile activity via activation of H1 and H2 but not H3 receptors.

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References

  1. Lindner HH. Embryology and anatomy of the biliary tree. In: Way LW, Pellegrini CA, editors. Gallbladder and Bile Ducts, Philadelphia: Saunders, 1987:3–22.

    Google Scholar 

  2. Johnston J. National Institute of Diabetes and Digestive and Kidney Diseases. J NIH Res 1990;2:50–2.

    Google Scholar 

  3. Ryan JP. Motility of the gallbladder and biliary tree. In: Johnson L, editor. Physiology of the Gastrointestinal Tract, New York: Raven Press, 1987:695–721.

    Google Scholar 

  4. Thornell E, Jansson R, Svanvik J. Indomethacin intravenously — A new way for effective relief of biliary pain: A double-blind study in man. Surgery 1981;90:468–72.

    PubMed  Google Scholar 

  5. Thornell E, Nilsson B, Jansson R, Svanik J. Effect of shortterm indomethacin treatment on the clinical course of acute obstructive cholecystitis. Eur J Surg 1991;157:127–30.

    PubMed  Google Scholar 

  6. Lorenz W, Schauer A, Heitland S, Calvoer R, Werle E. Biochemical and histochemical studies in the distribution of histamine in the digestive tract of man, dog and other mammals. Naunyn-Schmiedebergs Arch Pharmak 1969;265:81–100.

    Google Scholar 

  7. Lorenz W, Matejka E, Schmal A, Weidel W, Reimann H-J, Uhlig R, et al. A phylogenetic study on the occurrence and distribution of histamine in the gastro-intestinal tract and other tissues of man and various animals. Comp gen Pharmac 1973;4:229–50.

    Google Scholar 

  8. Lorenz W, Parkin JV, Rohde H, Barth H, Troidl H, Thon K, et al. Histamine in gastric secretory disorders: The relevance of the mucosal histamine content and the origin of histamine in gastric aspirate. In: Konturek SJ, Domschke W, editors. Gastric Secretion: Basic and Clinical Aspects. New York: Georg Thieme Verlag, 1981:29–51.

    Google Scholar 

  9. Lindlar R, Schäfer U, Lorenz W, Sattler J, Schröeder D, Krack W. Evidence for reduced traumatization during laparoscopic versus conventional cholecystectomy: Different changes in histamine levels related to special phases of operation. Agents Actions 1992;C162–7.

  10. Bauer H. Die Gallenblase als Streßorgan. Ein Beitrag zur Pathogenese der post-traumatischen Cholecystitis. Chirurg 1984;55:828–9.

    PubMed  Google Scholar 

  11. Arunlakshana O, Schild HO. Some quantitative uses of drug antagonists. Br J Pharmacol Chemother 1959;27:427–39.

    Google Scholar 

  12. Black JW, Duncan WAM, Durant CJ, Ganellin CR, Parsons ME. Definition and antagonism of histamine H2-receptors. Nature 1972;236:385–90.

    PubMed  Google Scholar 

  13. Arrang JM, Garbarg M, Schwartz JC. Auto-inhibition of brain histamine release mediated by a novel class (H3) of histamine receptors. Nature 1983;302:832–7.

    PubMed  Google Scholar 

  14. Barnes PJ, Ichinose M. H3-receptors in airways. TIPS 1989;10:264.

    PubMed  Google Scholar 

  15. Ichinose M, Barnes PJ. Histamine H3-receptors modulate nonadrenergic noncholinergic neural bronchoconstriction in guinea-pig in-vivo. Eur J Pharmacol 1989;163:383–6.

    PubMed  Google Scholar 

  16. Tamura K, Palmer JM, Wood JD. Presynaptic inhibition produced by histamine at nicotinic synapses in enteric ganglia. Neurosci 1988;25:171–9.

    Google Scholar 

  17. Leurs R, Brozius MM, Smit MJ, Bast A, Timmerman H. Effects of histamine H1-, H2- and H3-receptor selective drugs on the mechanical activity of guinea-pig small and large intestine. Br J Pharmacol 1991;102:179–85.

    PubMed  Google Scholar 

  18. Mack A, Todd J. A study of human gall bladder muscle in vitro. Gut 1968;9:546–9.

    PubMed  Google Scholar 

  19. Wise WE, LaMorte WW, Gaca JM, Schoetz DJ, Birkett DH, Williams LF. Reciprocal H1- and H2-histamine receptors in guinea-pig gallbladder. J Surg Res 1982;33:146–50.

    PubMed  Google Scholar 

  20. Waldman D, Zfass A, Makhlouf G. Stimulatory (H1) and inhibitory (H2) receptors in gallbladder muscle. Gastroenterol 1977;72:932–6.

    Google Scholar 

  21. Impicciatore M. Occurrence of H1- and H2-histamine receptors in the guinea-pig gallbladder in-situ. Br J Pharmacol 1978;64:291–22.

    Google Scholar 

  22. Ekblad E, Wahlestedt C, Hakanson R, Sundler F, Watanabe T, Wada H. Is histamine a neurotransmitter in the gut? Evidence from histidine decarboxylase immunocytochemistry. Acta Physiol Scand 1985;123:225–7.

    PubMed  Google Scholar 

  23. Hakanson R, Wahlestedt C, Westlin L, Vallgren S, Sundler F. Neuronal histamine in the gut wall releasable by gastrin and cholecystokinin. Neurosci Lett 1983;42:305–10.

    PubMed  Google Scholar 

  24. Mawe GM. Intracellular recording from neurones of the guinea-pig gall-bladder. J Physiol (Lond) 1990;429:323–38.

    Google Scholar 

  25. Jennings LJ, Malek N, Gimeno AM, Pozo M-J, Singh J, Salido GM, Davison JS. Histaminergic control of contraction of the isolated guinea-pig gallbladder. J Physiol (Lond) 1991;446:195P.

    Google Scholar 

  26. Ganellin CR. Chemistry and structure-activity relationships of drugs acting at histamine receptors. In: Ganellin CR, Parsons ME, editors. Pharmacology of Histamine Receptors. Bristol: Wright, 1982:10–102.

    Google Scholar 

  27. Hill S. Distribution, properties and functional characteristics of three classes of histamine receptor. Pharmacol Rev 1990;42:45–83.

    PubMed  Google Scholar 

  28. Lennon F, Feeley TM, Clanachan AS, Scott GW. Effects of histamine receptor stimulation on diseased gallbladder and cystic duct. Gastroenterol 1984;87:257–62.

    Google Scholar 

  29. Berthoud H-R, Jedrzejewska A, Powley TL. Simultaneous labeling of vagal innervation of the gut and afferent projections from the visceral forebrain with dil injected into the dorsal vagal complex of the rat. J Comp Neurol 1990;301:65–79.

    PubMed  Google Scholar 

  30. Mawe GM, Gokin AP, Wells DG. Actions of cholecystokinin and norepinephrine on vagal inputs to ganglion cells in guineapig gallbladder. Am J Physiol 1994;267:G1146–51.

    PubMed  Google Scholar 

  31. Mawe GM. The role of cholecystokinin in ganglionic transmission in the guinea-pig gall-bladder. J Physiol (Lond) 1991;439:89–102.

    Google Scholar 

  32. Mawe GM. Noradrenaline acts as a presynaptic inhibitory neurotransmitter in ganglia of the guinea-pig gall-bladder. J Physiol (Lond) 1993;461:378–402.

    Google Scholar 

  33. Wood JD. Histamine signals in enteric neuro-immune interactions. Ann NY Acad Sci 1992;664:275–83.

    PubMed  Google Scholar 

  34. Frieling T, Cooke HJ, Wood JD. Histamine receptors on submucous neurons in guinea-pig colon. Am J Physiol 1994;264:G74–80.

    Google Scholar 

  35. Cook HJ. Neural and Humoral regulation of the Small Intestinal Electrolyte Transport. In: Johnson LR, editor. Physiology of the Gastrointestinal Tract. New York: Raven Press, 1987:1307–50.

    Google Scholar 

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Author notes

  1. L. J. Jennings

    Present address: Department of Anatomy and Neurobiology, University of Vermont, College of Medicine, VT05405, Burlington, USA

Authors and Affiliations

  1. Department of Applied Biology, University of Central Lancashire, PR1 2HE, Preston, Lancashire, UK

    L. J. Jennings, R. W. Lea & J. Singh

  2. Department of Physiology, Faculty of Veterinary Science, University of Extremadura, E-10071, Caceres, Spain

    G. M. Salido & M. -J. Pozo

  3. Department of Medical Physiology, University of Calgary, T2N 4N1, Calgary, Alberta, Canada

    J. S. Davison & K. A. Sharkey

Authors
  1. L. J. Jennings
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  2. G. M. Salido
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  3. M. -J. Pozo
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  4. J. S. Davison
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  6. R. W. Lea
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  7. J. Singh
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Jennings, L.J., Salido, G.M., Pozo, M.J. et al. The source and action of histamine in the isolated guinea-pig gallbladder. Inflamm Res 44, 447–453 (1995). https://doi.org/10.1007/BF01757702

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  • DOI: https://doi.org/10.1007/BF01757702

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