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Peanut bud necrosis tospovirus S RNA: complete nucleotide sequence, genome organization and homology to other tospoviruses

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Summary

The complete nucleotide sequence of the S RNA of peanut bud necrosis virus (PBNV) has been determined. The RNA is 3 057 nucleotides in length, contains inverted repeats and two open reading frames (ORFs) with an ambisense coding strategy that are separated by an A+U-rich intergenic region. One ORF (1 320 nucleotides in the viral sense strand) encodes a Mr 49.5 kDa protein, identified as the nonstructural (NSs) protein based on similarity to published tospovirus sequences. The second ORF (831 nucleotides in virus complementary strand) encodes a Mr 30.6 kDa protein. This protein was identified as the nucleocapsid (N) protein based on sequence similarities. Amino acid sequence comparison of N and NSs proteins revealed identities of 22–34% with the reported tospovirus isolates of serogroups I, II, and III, whereas it had 82–86% identity with viruses in serogroup IV, watermelon silver mottle virus (WSMV) and tomato isolate of peanut bud necrosis virus (PBNV-To). Two subgenomic RNA species detected in PBNV infected tissue corresponded to the predicted sizes (1.65 and 1.4 kb) of the NSs and N mRNAs. The data presented show conclusively that PBNV should be included in serogroup IV, along with WSMV and PBNV-To.

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Authors and Affiliations

  1. Crop Protection Division, International Crops Research Institute for the Semi-Arid Tropics Asia Center (ICRISAT-IAC), Patancheru, India

    T. Satyanarayana, D. V. R. Reddy & R. A. Naidu

  2. Plant Genetic Resources Conservation Unit, USDA, USA

    S. E. Mitchell, S. Brown, S. Kresovich & R. Jarret

  3. Department of Plant Pathology, The University of Georgia, Georgia Experiment Station, Griffin, Georgia, USA

    J. W. Demski

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  1. T. Satyanarayana
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  2. S. E. Mitchell
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  3. D. V. R. Reddy
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  4. S. Brown
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  7. R. A. Naidu
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  8. J. W. Demski
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Satyanarayana, T., Mitchell, S.E., Reddy, D.V.R. et al. Peanut bud necrosis tospovirus S RNA: complete nucleotide sequence, genome organization and homology to other tospoviruses. Archives of Virology 141, 85–98 (1996). https://doi.org/10.1007/BF01718590

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  • DOI: https://doi.org/10.1007/BF01718590

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