Summary
Here we describe an African swine fever virus (ASFV) protein encoded by the open reading frame 5-AR that shares structural and functional similarities with the family of bacterial histone-like proteins which include histone-like DNA binding proteins, integration host factor, andBacillus phage SPO1 transcription factor, TF1. The ASFV 5-AR gene was cloned by PCR and expressed inE. coli. Monospecific antiserum prepared to the 5-AR bacterial expression product specifically immunoprecipitated a protein of approximately 11.6 kDa from ASFV infected swine macrophages at late times post-infection. Additionally, the 5-AR expression product was strongly recognized by ASFV convalescent pig serum, indicating its antigenicity during natural infection. Cloned p11.6 bound both double and single stranded DNA-cellulose columns. Consistent with a DNA binding function, immunoelectronmicroscopy localized p11.6 to the virion nucleoid. To our knowledge, p11.6 is the first bacterial histone-like DNA-binding protein found in an animal virus or eukaryotic cell system.
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Borca, M.V., Irusta, P.M., Kutish, G.F. et al. A structural DNA binding protein of African swine fever virus with similarity to bacterial histone-like proteins. Archives of Virology 141, 301–313 (1996). https://doi.org/10.1007/BF01718401
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DOI: https://doi.org/10.1007/BF01718401