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Comparative efficacy of ciprofloxacin, ceftazidime and gentamicin, given alone or in combination, in a model of experimental septicemia due to klebsiella pneumoniae in neutropenic mice

Therapeutische Aktivität von Ciprofloxacin, Ceftazidim und Gentamicin allein und in Kombination in einem Modell der experimentellen Klebsiella pneumoniae-Septikämie bei neutropenischen Mäusen

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Summary

The therapeutic efficacy of ciprofloxacin, used alone and in combination with either ceftazidime or gentamicin, was evaluated in a model of experimentalKlebsiella pneumoniae septicemia in neutropenic mice. Therapeutic results were compared to those achieved by treatment with ceftazidime or gentamicin alone, as well as their combination. Infections were induced by i.v. injection of two strains ofK. pneumoniae. Therapy with i.m. antibiotics was performed in 8 h intervals for a total of 13 doses, and survival rates were recorded at the end of treatment as well as seven days later. Ciprofloxacin alone proved to be significantly more effective than ceftazidime and gentamicin, producing survival rates similar to the combination of ceftazidime plus gentamicin. Bacterial counts of spleens and blood confirmed the superior bactericidal activity of ciprofloxacin. Except for the combination of ciprofloxacin with ceftazidime, there was no apparent advantage of combinations of ciprofloxacin with the other agents compared to ciprofloxacin alone. These data suggest a highin vivo activity of ciprofloxacin in systemic infection due toK. pneumoniae.

Zusammenfassung

Die therapeutische Aktivität von Ciprofloxacin in Monotherapie und in Kombination mit Ceftazidim oder Gentamicin wurde in einem Modell der experimentellenKlebsiella pneumoniae-Septikämie bei neutropenischen Mäusen untersucht. Zum Vergleich dienten Versuchsgruppen, die mit Ceftazidim oder Gentamicin jeweils allein oder in Kombination behandelt wurden. Die Infektion erfolgte durch i.v. Injektion von zwei verschiedenen Stämmen vonK. pneumoniae. Zur i.m. Therapie wurden 13 Dosen der Antibiotika achtstündlich injiziert. Die Überlebensraten wurden am Ende der Behandlungsperiode und sieben Tage später ermittelt. Die therapeutische Aktivität von Ciprofloxacin entsprach, gemessen an den Überlebensraten, der Kombination aus Ceftazidim und Gentamicin, war jedoch signifikant höher als die Wirksamkeit beider Einzelsubstanzen. Dies wurde auch durch die Ermittlung der Bakterienzahlen in Milz und Blut bestätigt. Mit Ausnahme der Kombination aus Ciprofloxacin und Ceftazidim kam es bei Ciprofloxacin-Kombinationen zu keiner Wirkungssteigerung. Diese Daten weisen auf eine hoheIn vivo-Aktivität von Ciprofloxacin bei systemischenK. pneumoniae-Infektionen hin.

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Authors and Affiliations

  1. Department of Internal Medicine, Klinikum Charlottenburg, Freie Universität Berlin, D-1000, Berlin

    M. Trautmann &  O. Brückner

  2. Institute of Medical Microbiology, Medizinische Universität zu Lübeck, D-2400, Lübeck 1

    R. Marre

  3. Institute of Medical Microbiology, Freie Universität Berlin, D-1000, Berlin

    H. Hahn

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  1. M. Trautmann
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  2. O. Brückner
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  3. R. Marre
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  4. H. Hahn
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Trautmann, M., Brückner, O., Marre, R. et al. Comparative efficacy of ciprofloxacin, ceftazidime and gentamicin, given alone or in combination, in a model of experimental septicemia due to klebsiella pneumoniae in neutropenic mice. Infection 16, 49–53 (1988). https://doi.org/10.1007/BF01646933

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