Summary
There exist only few data concerning the variation of oligosaccharides in human milk. In this study the variations of neutral oligosaccharides and of lactose in human milk during the feeding were determined from five women at day 8 and at day 57 post partum. The milk of the investigated feedings was divided in four parts of equal volumes during sampling; the concentrations of neutral oligosaccharide fractions were determined by gel permeation chromatography on Fractogel TSK HW 40 (S) columns. No significant differences in the concentrations of the neutral oligosaccharide groups monofucosyllactoses, difucosyllactose, lacto-N-tetraoses, monofucosyllacto-N-tetraoses and difucosyllacto-N-tetraoses and of lactose were found in the four milk parts. The results of this study favor the use of socalled mid-feed samples, a simple and convenient sampling method for analytical studies of human milk. Mid-feed samples are representative of the whole feeding as concerned for neutral oligosaccharides.
Zusammenfassung
Über die Variation von Oligosacchariden in Humanmilch liegen nur wenig Daten vor. In dieser Studie werden die Änderungen von neutralen Oligosacchariden und von Lactose während des Stillvorgangs bei 5 Frauen am Tag 8 und am Tag 57 nach der Geburt bestimmt. Die Milchproben der untersuchten Stillvorgänge wurden zu jeweils 4 Teilen gleichen Volumens gesammelt. Die Konzentration der neutralen Oligosaccharide wurde durch Gelchromatographie mit Fractogel TSK HW 40 (S) Säulen bestimmt. In den jeweils 4 Milchteilen der Stillvorgänge waren bei den Konzentrationen der neutralen Oligosaccharidgruppen Monofucosyllactosen, Difucosyllactose, Lacto-N-tetraosen, Monofucosyllacto-N-tetraosen und Difucosyllacto-N-tetraosen und bei Lactose keine signifikanten Unterschiede festzustellen. Die Ergebnisse dieser Studie stützen die sogenannte „mid-feed“ Sammeltechnik als eine einfache und praktische Probennahmemethode für analytische Milchstudien. Die „mid-feed“ Proben sind bezüglich der Oligosaccharide repräsentativ für die gesamte Milch eines Stillvorganges.
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References
Andersson B, Porras O, Hanson LA, Lagergard T, Svanborg-Eden C (1986) Inhibition of attachment of Streptococcus pneumoniae and Haemophilus influenzae by human milk and receptor oligosaccharides. J Infect Diseases 153:232–237
Ashkenazi S, Mirelman D (1987) Nonimmunoglobulin fraction of human milk inhibits the adherence of certain enterotoxigenic Escherichia coli strains to guinea pig intestinal tract. Pediatric Research 22:130–134
Bezkorovainy A (1989) Ecology of bifidobacteria. In: Bezkorovainy A, Miller-Catchpole R (eds) Biochemistry and physiology of bifidobacteria. Boca Raton: CRC Press, pp 30–72
Bruntz R, Dabrowski U, Dabrowski J, Ebersold A, Peter-Katalinic J, Egge H (1988) Fucose-containing oligosaccharides from a donor of blood group 0 Lea nonsecretor. Biol Chem Hoppe-Seyler 369:257–273
Bundesministerium für Jugend, Familie, Frauen und Gesundheit (1987) Revidierte Deklaration von Helsinki. Bundesanzeiger 108:159–163
Coppa GV, Gabrielli O, Giorgi P, Catassi C, Montanari MP, Varaldo PE, Nichols BL (1990) Preliminary study of breastfeeding and bacterial adhesion to uroepithelial cells. Lancet 335:569–571
Eglin RP, Wilkinson AR (1987) HIV infection and pasteurisation of breast milk. Lancet i:1093
Hibberd CM, Brooke OG, Carter ND, Haug M, Harzer G (1982) Variation in the composition of breast milk during the first 5 weeks of lactation: implications for the feeding of preterm infants. Arch Dis Child 57:658–662
Kobata A, Yamashita K, Tachibana Y (1978) Oligosaccharides from human milk. In: Ginsburg V (ed) Meth Enzymol 50:216–220
Montreuil J, Mullet S (1960) Etude des variations des constituants glucidiques du lait de femme au cours de la lactation. Bull Soc Chim Biol 42:365–377
Neville MC, Keller RP, Seacat J, Casey CE, Allen JC, Archer P (1984) Studies on human lactation. I. Within-feed and between-breast variation in selected components of human milk. Am J Clin Nutr 40:635–646
Strecker G, Wieruszeski JM, Michalski JC, Montreuil J (1989) Primary structure of human milk nona- and decasaccharides by a combination of fast atom bombardment mass spectrometry and1H/13C-nuclear magnetic resonance spectroscopy. Evidence for a new core structure, iso-lacto-N-octaose. Glycoconjugate J 6:169–182
Thurl S, Offermanns J, Müller-Werner B, Sawatzki G (1991) Determination of neutral oligosaccharide fractions from human milk by gel permeation chromatography. J Chromatogr Biomed Appl 568:291–300
Viverge D, Grimmonprez L, Cassanas G, Bardet L, Bonnet H, Solere M (1985) Variations of lactose and oligosaccharides in milk from women of blood types secretor A or H, secretor Lewis, and secretor H/nonsecretor Lewis during the course of lactation. Ann Nutr Metab 29:1–11
Viverge D, Grimmonprez L, Cassanas G, Bardet L, Solere M (1986) Diurnal variations and within the feed in lactose and oligosaccharides of human milk. Ann Nutr Metab 30:196–209
Viverge D, Grimmonprez L, Cassanas G, Bardet L, Solere M (1990) Variations in oligosaccharides and lactose in human milk during the first week of lactation. J Pediatr Gastroenterol Nutr 11:361–364
Wilcoxon F, Wilcox RA (1964) Some rapid approximate statistical procedures. In: Lederle Laboratories, Pearl River, New York
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Thurl, S., Henker, J., Taut, H. et al. Variations of neutral oligosaccharides and lactose in human milk during the feeding. Z Ernährungswiss 32, 262–269 (1993). https://doi.org/10.1007/BF01611164
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DOI: https://doi.org/10.1007/BF01611164