Abstract
S. cerevisiae was grown in a blackstrap molasses containing medium in batch and fed-batch cultures. The following parameters were varied: pH (from 4.0 to 6.5), dissolved oxygen (DO) (from 0 to 5.0 mg O2L−1) and sucrose feeding rate. When glucose concentration (S) was higher than 0.5 g L−1 a reduction in the specific invertase activity of intact cells (v) and an oscillatory behavior of v values during fermentation were observed. Both the invertase reduction and the oscillatory behavior of v values could be related to the glucose inhibitory effect on invertase biosynthesis. The best culture conditions for attainingS. cerevisiae cells suitable for invertase production were: temperature=30°C; pH=5.0; DO=3.3 mg O2L−1; (S)=0.5 g L−1 and sucrose added into the fermenter according to the equations: (V−Vo)=t2/16 or (V−Vo)=(Vf−Vo)·(e0.6t−1)/10.
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Vitolo, M., Duranti, M.A. & Pellegrim, M.B. Effect of pH, aeration and sucrose feeding on the invertase activity of intactS. cerevisiae cells grown in sugarcane blackstrap molasses. Journal of Industrial Microbiology 15, 75–79 (1995). https://doi.org/10.1007/BF01569803
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DOI: https://doi.org/10.1007/BF01569803