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The effects of quinine on the calcium and magnesium content of the sarcoplasmic reticulum and the temperature-dependence of quinine contractures

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Summary

A significant decrease in the Ca2+ and increase in the Mg2+ content of the terminal cisternae (TC) of the sarcoplasmic reticulum (SR) during quinine contraction was demonstrated by electron probe analysis of rapidly frozen frog muscles. The extent of Ca2+ release (71% of total) from the TC and the absence of an increase in total cell Ca2+ support the conclusion that quinine contractures are caused by passive efflux of Ca2+ from the SR when the latter is uncompensated due to inhibition of the SR Ca2+ pump by quinine. A rapid warming contraction (RWC) was observed, in the presence of quinine, when the temperature of intact and skinned muscles was increased from about 5° C to 18–23° C. The duration of the latency of quinine contracture, in intact muscle bundles, was approximately 31 s at 3° C and 2 s at 23° C. The results suggest a significant temperature sensitivity of the passive Ca2+ channels of the SR membrane, although an effect of temperature on the lipid partition coefficient of quinine into the SR has not been ruled out.

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Authors and Affiliations

  1. Department of Physiology, Tokai University School of Medicine, Isehara, 259-11, Kanagawa, Japan

    Toshitada Yoshioka

  2. Pennsylvania Muscle Institute, University of Pennsylvania School of Medicine, 19104, Philadelphia, Pennsylvania, USA

    Andrew P. Somlyo

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  1. Toshitada Yoshioka
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  2. Andrew P. Somlyo
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Yoshioka, T., Somlyo, A.P. The effects of quinine on the calcium and magnesium content of the sarcoplasmic reticulum and the temperature-dependence of quinine contractures. J Muscle Res Cell Motil 8, 322–328 (1987). https://doi.org/10.1007/BF01568888

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  • DOI: https://doi.org/10.1007/BF01568888

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