Abstract
The study was undertaken to examine regional differences in the concentrations of five regulatory peptides in the human colonic mucosa. Biopsies were obtained during routine colonoscopy from 33 patients whose colonic mucosa was macroscopically and histologically normal. Regulatory peptides were extracted, and measured by specific radioimmunoassays. Concentrations of three peptides that are present predominantly in endocrine cells within colonic mucosa increased significantly towards the rectum: Mean concentrations of peptide YY, enteroglucagon, and somatostatin were about three times greater in the rectum than in the cecum. However, concentrations of two peptides that are present in mucosal nerve fibers diminished significantly towards the rectum: Mean rectal concentrations of vasoactive intestinal peptide and peptide histidine methionine were both about 0.6 of mean cecal concentrations. Concentrations of all five peptides were lower in biopsies taken from colonic polyps than in normal colonic mucosa. Regional differences in colonic mucosal concentrations of regulatory peptides probably reflect differences in the physiological functions of different parts of the colon.
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Adrian TE, Savage AP, Bacarese-Hamilton AJ, Wolfe K, Besterman HS, Bloom SR: Peptide YY abnormalities in gastrointestinal diseases. Gastroenterology 90:379–384, 1986
Tari A, Miyachi Y, Sumii K, Kajiyama G, Miyoshi A: Peptide YY-like immunoreactivity in normal colon mucosa, muscle layer and adenocarcinoma. Jpn J Med 26:184–188, 1987
Bottcher G, Alumets J, Hakanson R, Sundler F: Coexistence of glicentin and peptide YY in colorectal L-cells in cat and man. An electron microscopic study. Regul Peptides 13:283–291, 1986
Lehy T, Peranzi GC: Correlative immunocytochemical and electron microscopic studies: Identification of (entero)glucagon-, somatostatin-, and pancreatic polypeptide-likecontaining cells in human colon. Histochemistry 71:67–80, 1981
Baldissera FG, Holst JJ, Jensen SL, Krarup T: Distribution and molecular forms of peptides containing somatostatin immunodeterminants in extracts from the entire gastrointestinal tract of pig and man. Biochim Biophys Acta 838:132–143, 1985
Larsson LI, Farenkrug J, Schaffalitzki de Muckadell OB, Sundler F, Hakanson R, Rehfeld JF: Localisation of vasoactive intestinal polypeptide (VIP) to central and peripheral neurons. Proc Natl Acad Sci 73:3197–3200, 1976
Tatemoto K, Jornvall H, McDonald TJ, Carlquist M, Go VL, Johansson C, Mutt V: Isolation and primary structure of human PHI (peptide HI). FEBS Lett 174:258–261, 1984
Adrian TE, Ferri G-L, Bacarese-Hamilton AJ, Fuessl HS, Polak JM, Bloom SR: Human distribution and release of a putative new gut hormone; peptide YY. Gastroenterology 89:1070–1077, 1985
Koch TR, Roddy DR, Go VLW: Abnormalities of fasting serum concentrations of peptide YY in the idiopathic inflammatory bowel diseases. Am J Gastroenterol 82:321–326, 1987
Yiangou Y, Williams SJ, Bishop AE, Polak JM, Bloom SR: PHM immunoreactivity in plasma and tissue from patients with VIP-secreting tumours and watery diarrhoea syndrome. J Clin Endocrinol Metab 64:131–139, 1987
Mutt V: Hormone isolation.In Gut Hormones, 1st ed. SR Bloom (ed). Edinburgh, Churchill Livingstone, 1978, pp 21–27
Bloom SR, Long RB (eds). Radioimmunoassay of Gut Regulatory Peptides. London, WB Saunders, 1982
Ghatei MA, Uttenthal LO, Christofides ND, Bryant MG, Bloom SR: Molecular forms of human enteroglucagon in tissue and plasma: Responses to nutrient stimuli in health and disorders of the upper gastrointestinal tract. J Clin Endocrinol Metab 57:488–495, 1983
Adrian TE, Bacarese-Hamilton AJ, Smith HA, Chohan P, Manolas KJ, Bloom SR: Distribution and postprandial release of porcine peptide YY. J Endocrinol 113:11–14, 1987
O'Shaughnessy DJ: Somatostatin.In Radioimmunoassay of Gut Regulatory Peptides. SR Bloom, RG Long (eds). London, WB Saunders, 1982, pp 138–145
Mitchell SJ, Bloom SR: Measurement of fasting and postprandial plasma VIP in man. Gut 19:1043–1048, 1978
Bloom SR, Polak JM (eds). Gut Hormones, 2nd ed. Edinburgh, Churchill Livingstone, 1981
Bishop AE, Polak JM, Bryant MG, Bloom SR, Hamilton S: Abnormalities of vasoactive intestinal polypeptide-containing nerves in Crohn's disease. Gastroenterology 79:853–860, 1980
O'Morain C, Bishop AE, McGregor GP, Levi AJ, Bloom SR, Polak JM, Peters TJ: Vasoactive intestinal peptide concentrations and immunocytochemical studies in rectal biopsies from patients with inflammatory bowel disease. Gut 25:57–61, 1984
Koch TR, Carney JA, Go VL: Distribution and quantitation of gut neuropeptides in normal intestine and inflammatory bowel disease. Dig Dis Sci 32:369–376, 1987
Sjolund K, Schaffalitzky nde Muckadell OB, Farenkrug J, Hakanson R, Peterson BG, Sundler F: Peptide-containing nerve fibers in the gut wall in Crohn's disease. Gut 24:724–733, 1983
Van Den Ingh HF, Van Den Broek LJ, Verhoestad AA, Bosman FT: Neuroendocrine cells in colorectal adenomas. J Pathol 148:231–237, 1986
Gibbs NM: Incidence and significance of argentaffin and Paneth cells in some tumours of the large intestine. J Clin Pathol 20:826–831, 1967
Kaye GI, Fenoglio CM, Pascal R, Lane N: Comparative electron microscopic features of normal, hyperplastic, and adenomatous human colonic epithelium. Gastroenterology 64:926–945, 1973
Donkier J, McGregor G, Impallomeni M, Calam J, Bloom S: Age related changes in regulatory peptides in rectal mucosa. Acta Gastoenterol Belg 50:405–410, 1987
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Calam, J., Ghatei, M.A., Domin, J. et al. Regional differences in concentrations of regulatory peptides in human colon mucosal biopsy. Digest Dis Sci 34, 1193–1198 (1989). https://doi.org/10.1007/BF01537267
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DOI: https://doi.org/10.1007/BF01537267