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Effect of a new synthetic serum replacement on insulin and somatostatin secretion from isolated rat pancreatic islets in long-term culture

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Journal of tissue culture methods

Summary

Serum contains insulin degrading components. We have evaluated the insulin and somatostatin secretion from isolated rat pancreatic islets during a 2-wk culture period using three different serum-containing media, and one serum-free medium with a synthetic serum replacement. Islets incubated in serum-free medium elicited significantly higher daily insulin and somatostatin secretions than islets incubated in the serum-containing media. After a 2-wk culture period, islets from the serum-free medium secreted significantly more insulin and somatostatin than islets cultured in other media when stimulated with 25 mmol/liter glucose together with 15 mmol/liter theophylline. We conclude that the serum-free medium is superior for long-term culture of rat pancreatic islets.

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References

  1. Andersson, A. Isolated mouse pancreatic islets in culture: effects of serum and different culture media on the insulin production of the islets. Diabetologia 14:397–404; 1978.

    PubMed  Google Scholar 

  2. Andersson, A.; Hellerstrøm, C. Metabolic characteristics of isolated pancreatic islets in tissue culture. Diabetes 21:546–554; 1972.

    PubMed  Google Scholar 

  3. Bertheussen, K.; Holst, N.; Forsdahl, F., et al. A new cell culture assay for quality control in IVF. Hum. Reprod. 4:531–535; 1989.

    PubMed  Google Scholar 

  4. Goldman, H.; Colle, E. Human pancreatic islets in culture: effects of supplementing the medium with homologous and heterologous serum. Science 192:1014–1016; 1976.

    PubMed  Google Scholar 

  5. Hansen, T.; Hannestad, K. Simple rosette assay for HLA-B27 typing of whole blood samples. Tissue Antigens 30:198–203; 1987.

    PubMed  Google Scholar 

  6. Hetland, G.; Johnson, E.; Eskeland, T. Formation of the membrane attack complex of complement (MAC) on erythrocytes from monocyte-produced terminal complement components. Scand. J. Immunol. 25:571–577; 1987.

    PubMed  Google Scholar 

  7. Jorde, R.; Burhol, P. G.; Schultz, T. B., et al. The effect of a 34-h fast on the meal-induced rises in plasma GIP, serum insulin, and blood glucose in man. Scand. J. Gastroenterol. 16:109–112; 1981.

    PubMed  Google Scholar 

  8. Kedinger, M.; Moody, A.; Launay, J. F., et al. Establishment of serum based medium essentially free of proteolytic activity for the culture of mouse pancreatic islets. Experientia 33:972–973; 1977.

    PubMed  Google Scholar 

  9. Kostianovsky, M.; Lacy, P. E.; Greider, M. H., et al. Long term (15 days) incubation of islets of Langerhans isolated from adult rats and mice. Lab. Invest. 27:53–61; 1972.

    PubMed  Google Scholar 

  10. Maurer, H. R. Towards chemically-defined, serum free media for mammalian cell culture. In: Freshney, R. I., ed. Animal cell culture. Oxford: IRL Press; 1986:13–31.

    Google Scholar 

  11. Malaisse, W. J.; Kawazu, S.; Herchuelz, A., et al. The stimulus secretion coupling of glucose-induced insulin release: effect of lactate upon islet function. Arch. Biochem. Biophys. 194:49–62; 1979.

    PubMed  Google Scholar 

  12. Niclsen, J. H.; Eff, C.; Deckert, T., et al. Effect of diabetic sera on isolated islets in tissue culture. Diabetologia 13:421; 1977.

    Google Scholar 

  13. Nielsen, J. H. Growth and function of the pancreatic β cell in vitro. Acta Endocrinol. 108(Suppl):266; 1985.

    PubMed  Google Scholar 

  14. Sener, A.; Kawazu, S.; Hutton, J. C., et al. The stimulus-secretion coupling of glucose-induced insulin release: effect of exogenous pyruvate on islet function. Biochem. J. 176:217–32; 1978.

    PubMed  Google Scholar 

  15. Verspohl, E. J.; Kaiser, P.; Wahl, M., et al. Decreased insulin secretory response of pancreatic islets during culture in the presence of low glucose is associated with diminished 45Ca2+ net uptake, NADPH/NADP+ and GSH/GSSG ratios. Life Sci. 43:209–219; 1988.

    PubMed  Google Scholar 

  16. Vonen, B.; Florholmen, J.; Giaever, A. K., et al. A methodological study of discontinuous Percoll-gradient separation of pancreatic islet from rats. Scand. J. Clin. Lab. Invest. 47:415–420; 1987.

    PubMed  Google Scholar 

  17. Vonen, B.; Florholmen, J.; Giæver, A. K., et al. Radioimmunoassay of somatostatin from isolated rat pancreatic islets. Scand. J. Clin. Lab. Invest. 49:135–138; 1989.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Gastroenterology, Department of Medicine, University Hospital of Tromsø, Tromsø, Norway

    Barthold Vonen, Anton K. Giæver, Jon Florholmen & Per G. Burhol

  2. Department of Surgery, University Hospital of Tromsø, Tromsø, Norway

    Barthold Vonen

  3. Institute of Medical Biology, University of Tromsø, Tromsø, Norway

    Kjell Bertheussen

Authors
  1. Barthold Vonen
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  2. Kjell Bertheussen
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  3. Anton K. Giæver
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  4. Jon Florholmen
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  5. Per G. Burhol
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Vonen, B., Bertheussen, K., Giæver, A.K. et al. Effect of a new synthetic serum replacement on insulin and somatostatin secretion from isolated rat pancreatic islets in long-term culture. Journal of Tissue Culture Methods 14, 45–50 (1992). https://doi.org/10.1007/BF01404743

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  • DOI: https://doi.org/10.1007/BF01404743

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