Summary
Human female lymphocytes were exposed to X-rays in vitro at 7 different doses between 40–280 R. In 830 metaphases chromosome analyses were carried out with either conventional staining or G-banding, respectively. 486 breakpoints are non-randomly distributed between chromosomes and chromosome arms. An excess of lesions was present in chromosomes 1 and 5 or in lp. 85% of the lesions were located in G-negative bands (pale G-bands). 29% of all lesions appeared in either the last terminal pale band (21%) or in the centromere region (8%).
With regard to an application of G-banding for a biological dose-estimation, the dose-response relations of dic and ace were analysed. Although G-banding enables detailed analysis of the whole karyotype it cannot be recommended for cytogenetic routine analyses in medical radioprotection monitoring, without suitable automated scoring techniques. Dose estimations based on the frequency of dic and carried out with conventional staining cannot be essentially improved at present with banding. Nevertheless, by banding criteria for a correct evaluation of other aberration types, e.g. ace, can be provided. This is a prerequisite for the calculation of representative dose-effect curves.
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References
Bender, M. A., Gooch, P. S.: Types and rates of X-ray induced chromosome aberrations in human blood irradiated in vitro. Proc. Natl. Acad. Sci.48, 522–532 (1962)
Buckton, K. E.: Identification with G- and R-banding of the position of breakage points induced in human chromosomes by in vitro X-irradiation. Int. J. Radiat. Biol.29, 475–488 (1976)
Caspersson, T., Haglund, V., Lindell, B., Zech, L.: Radiation induced non random chromosome breakage. Exp. Cell Res.75, 541–543 (1972)
Cooke, P., Seabright, M., Wheeler, M.: The differential distribution of X-ray induced chromosome lesions in trypsin banded preparations from human subjects. Humangenetik28, 221–231 (1975)
Dubos, C., Pequignot, E. V., Dutrillaux, B.: Localisation ofγ-rays induced chromatid breaks using a three consecutive staining technique. Mutat. Res.49, 127–131 (1978)
Evans, H. J.: Chromosome aberrations induced by ionizing radiation. In Internat. Rev. Cytol.13, 221–231, Bourne, G. H., Danielli, J. F. (eds.), New York and London: Academic Press 1962
Feller, W.: An introduction to probability theory and its applications,2, 2nd ed. New York, London, Sydney, Toronto: Wiley J. and Sons, Inc. 1971
Heddle, J. A.: Randomness in the formation of radiationinduced chromosome aberrations. Genetics52, 1329–1334 (1965)
Holmberg, M., Jonasson, J.: Preferential location of X-ray induced chromosome breakage in the R-bands of the human chromosomes. Hereditas74, 57–68 (1973)
Holmberg, M., Jonasson, J.: Synergistic effect of X-ray and UV-irradiation on the frequency of chromosome breakage in human lymphocytes. Mutat. Res.23, 213–221 (1974)
Jonasson, J. Holmberg, M.: Evidence for an inverse relationship between X-ray induced chromatid and chromosome breakage in human chromosomes. Hereditas75, 259–266 (1973)
Kucerova, M., Polikova, Z.: Banding technique used for the detection of chromosomal aberrations induced by radiation and alkylating agents. Mutat. Res.34, 279–290 (1976)
Mäkinen, T., Stenstrand, K., Selander, R. K.: The effect of the ionic strength on G-bands. Humangenetik28, 71–73 (1975)
Paris Conference (1971): Standardization in Human Cytogenetics: Birth Defects, Original Article Series,8, No. 7 (New York: The National Foundation) (1972)
San Roman, C., Bobrow, M.: The sites of radiation induced breakage in human lymphocyte chromosomes, determined by quinacrine fluorescence. Mutat. Res.18, 325–331 (1973)
Savage, J. R. K.: Application of chromosome banding technique to the study of primary chromosome structural changes. J. Med. Genet.14, 362–370 (1977a)
Savage, J. R. K.: Asignment of aberration breakpoints in banded chromosomes. Nature270, 513–514 (1977b)
Schmid, E., Bauchinger, M., Hug, O.: Chromosomenaberrationen menschlicher Lymphocyten nach Röntgenbestrahlung in vitro. I. Qualitative und quantitative Aspekte der Dosis-Wirkungsbeziehung. Mutat. Res.16, 307–317 (1972)
Schmid, E., Bauchinger, M., Bunde, E., Ferbert, H. F., Lieven, H. v.: Comparison of the chromosome damage and its dose response after medical whole-body exposure to 60-Co-Gamma rays and irradiation of blood in vitro. Int. J. Radiat. Biol.26, 31–37 (1974)
Schmid, E., Bauchinger, M., Mergenthaler, W.: Analysis of the time relationship for the interaction of X-ray-induced primary breaks in the formation of dicentric chromosomes. Int. J. Radiat. Biol.30, 339–346 (1976)
Schneeberger, H.: Linearitätsteste der k-dimensionalen Regressionsgleichung und Anwendungen. Monatshefte für Mathematik64, 361–373 (1960)
Seabright, M.: High resolution studies on the pattern of induced exchanges in the human karyotype. Chromosoma40, 333–346 (1973a)
Seabright, M.: Noninvolvement of the human X chromosome in X-ray induced exchanges. Cytogenet. Cell Genet.12, 342–356 (1973)
Shiraishi, Y., Holdsworth, R. N., Minowada, J., Sandberg, A. A.: Specificity of chromosomal changes induced with X-rays in a human T-cell line. Rad. Res.73, 452–463 (1978)
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Bauchinger, M., Götz, G. Distribution of radiation induced lesions in human chromosomes and dose-effect relation analysed with G-banding. Radiat Environ Biophys 16, 355–366 (1979). https://doi.org/10.1007/BF01340573
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DOI: https://doi.org/10.1007/BF01340573