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The complete nucleotide sequence and genome organization of odontoglossum ringspot tobamovirus RNA

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Summary

The complete nucleotide sequence of the genomic RNA of odontoglossum ringspot tobamovirus (ORSV) was determined. The RNA genome of ORSV is 6618 nucleotides long and contains five open reading frames (ORFs 1 to 5) coding for proteins of Mr 126 K, 181 K, 34 K, 18 K and 52 K, respectively. This is the longest RNA of the known viruses of theTobamovirus genus. The sequences of the ORSV RNA encoded proteins exhibit high homology to the proteins of the members of theTobamovirus genus. The genomic organization and sequence analysis showed that ORSV is more closely related to tobacco mild green mosaic virus (TMGMV), pepper mild mottle virus (PMMV), tomato mosaic virus (ToMV) and TMV than to cucumber green mottle mosaic virus (CGMMV) and sunn-hemp mosaic virus (SHMV).

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References

  1. Alonso E, Garcia-Luque I, de la Cruz A, Wicke B, Avila-Rincon MJ, Serra MT, Castresana C, Diaz-Ruiz JR (1991) Nucleotide sequence of the genomic RNA pepper mild mottle virus, a resistance-breaking tobamovirus in pepper. J Gen Virol 72: 2875–2884

    Google Scholar 

  2. Argos P (1988) A sequence motif in many polymerases. Nucleic Acids Res 16: 9909–9916

    Google Scholar 

  3. Avila-Rincon MJ, Ferrero ML, Alonso E, Garcia-Luque I, Diaz-Ruiz JR (1989) Nucleotide sequences of 5′ and 3′ non-coding regions of pepper mild mottle virus strain S RNA. J Gen Virol 70: 3025–3031

    Google Scholar 

  4. Bruenn JA (1991) Relationships among the positive strand and double-strand RNA viruses as viewed through their RNA-dependent RNA polymerases. Nucleic Acids Res 19: 217–226

    Google Scholar 

  5. Calder VL, Palukaitis P (1992) Nucleotide sequence analysis of the movement genes of resistance breaking strains of tomato mosaic virus. J Gen Virol 73: 165–168

    Google Scholar 

  6. Carr JP, Marsh LE, Lomonossoff GP, Sekiya ME, Zaitlin M (1992) Resistance to tobacco mosaic virus induced by the 54-kDa gene sequence requires expression of the 54-kDa protein. Mol Plant Microbe Interact 5: 397–404

    Google Scholar 

  7. Culver JN, Lehto K, Close SM, Hilf ME, Dawson WO (1993) Genomic position affects the expression of tobacco mosaic virus movement and coat protein genes. Proc Natl Acad Sci USA 90: 2055–2059

    Google Scholar 

  8. Deom O, Oliver MJ, Beachy RN (1987) The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement. Science 237: 389–394

    Google Scholar 

  9. Dubs MC, Van Regenmortel MHV (1990) Odontoglossum ringspot virus coat protein: sequence and antigenic comparisons with other tobamoviruses. Arch Virol 115: 239–249

    Google Scholar 

  10. Edwardson JR, Zettler FW (1986) Odontoglossum ringspot virus. In: Van Regenmortel MHV, Fraenkel-Conrat H (eds) The plant viruses, vol 2. Plenum Press, New York, pp 233–247

    Google Scholar 

  11. Gallie DR, Sleat DE, Watts JW, Turner PC, Wilson TMA (1987) The 5′ leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcriptsin vitro andin vivo. Nucleic Acids Res 15: 3257–3273

    Google Scholar 

  12. Gallie DR, Sleat DE, Watts JW, Turner PC, Wilson TMA (1988) Mutational analysis of the tobacco mosaic virus 5′ leader sequence for altered ability to enhance translation. Nucleic Acids Res 16: 883–893

    Google Scholar 

  13. Garcia-Arenal F (1988) Sequence and structure at the genome 3′ end of the U2-strain of tobacco mosaic virus, a histidine-accepting tobamovirus. Virology 166: 201–206

    Google Scholar 

  14. Gibbs AJ (1986) Tobamovirus classification. In: Van Regenmortel MHV, Fraenkel-Conrat H (eds) The plant viruses, vol 2. Plenum Press, New York, pp 167–180

    Google Scholar 

  15. Goelet P, Lomonossoff GP, Butler PJG, Akam ME, Gait MJ, Karn J (1982) Nucleotide sequence of tobacco mosaic virus RNA. Proc Natl Acad Sci USA 79: 5818–5822

    Google Scholar 

  16. Gubler U, Hoffman BJ (1983) A simple and very efficient method for generating cDNA libraries. Gene 25: 263–269

    Google Scholar 

  17. Habili N, Symons RH (1989) Evolutionary relationship between luteoviruses and other RNA plant viruses based on sequence motifs in their putative RNA polymerases and nucleic acid helicases. Nucleic Acids Res 17: 9543–9555

    Google Scholar 

  18. Helmut B, Cribb P, Launert E (1992) The manual of cultivated orchid species, 3rd ed. American Orchid Society, Florida

    Google Scholar 

  19. Hodgman TC (1988) A new superfamily of replicative proteins. Nature 335: 22–23

    Google Scholar 

  20. Isomura Y, Matumoto Y, Murayama A, Chatani M, Inouye N, Ikegami M (1991) Molecular cloning, sequencing and expression inEscherichia coli of the odontoglossum ringspot virus coat protein gene. J Gen Virol 72: 2247–2249

    Google Scholar 

  21. Jensen DD, Gold AH (1951) A virus ring spot ofOdontoglossum orchids: symptoms, transmission, and electron microscopy. Phytopathology 41: 648–653

    Google Scholar 

  22. Konarska MM, Padgett RA, Sharp PA (1984) Recognition of cap structure in splicingin vitro of mRNA precursors. Cell 38: 731–736

    Google Scholar 

  23. Kukla BA, Guilley HA, Jonard GX, Richards KE, Mundry KW (1979) Characterization of long guanosine-free RNA sequences from Dahlamense and U2 strains of tobacco mosaic virus. Euro J Biochem 98: 61–66

    Google Scholar 

  24. MacFarlane SA, Davies JW (1992) Plants transformed with a region of the 201-kilodalton replicase gene from pea early browning virus RNA1 are resistant to virus infection. Proc Natl Acad Sci USA 89: 5829–5833

    Google Scholar 

  25. Meshi T, Ohno T, Iba H, Okada Y (1981) Nucleotide sequence of a cloned cDNA copy of TMV (cowpea strain) RNA, including the assembly origin, the coat protein cistron, and the 3′ noncoding region. Mol Gen Genet 184: 20–25

    Google Scholar 

  26. Meshi T, Ohno T, Okada Y (1982) Nucleotide sequence of the 30 K protein cistron of cowpea strain of tobacco mosaic virus. Nucleic Acids Res 10: 6111–6117

    Google Scholar 

  27. Mushegian AR, Koonin EV (1993) Cell-to-cell movement of plant viruses. Arch Virol 133: 239–257

    Google Scholar 

  28. Ohno T, Aoyagi M, Yamanashi Y, Saito H, Ikawa S, Meshi T, Okada Y (1984) Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome. J Biochem 96: 1915–1923

    Google Scholar 

  29. Padgett HS, Beachy RN (1993) Analysis of tobacco mosaic virus strain capable of overcoming N gene-mediated resistance. Plant Cell 5: 577–586

    Google Scholar 

  30. Park WM, Yoon KE, Chung SY, Ryu KH (1990) Identification of odontoglossum ringspot virus isolated fromCymbidium goeringii Reichenbach in Korea. Korean J Plant Pathol 6: 387–392

    Google Scholar 

  31. Park WM, Yoon KE, Chung SY, Ryu KH (1990) Purification and serological detection of odontoglossum ringspot virus isolated fromCymbidium goeringii in Korea. Korean J Plant Pathol 6: 474–481

    Google Scholar 

  32. Richards K, Jonard G, Guilley H, Keith G (1977) Leader sequence of 71 nucleotides devoid of G in tobacco mosaic virus RNA. Nature 267: 548–550

    Google Scholar 

  33. Rietveld K, Linschooten K, Pleij CWA, Bosch L (1984) The three dimensional folding of the tRNA-like structure of tobacco mosaic virus RNA: a new building principle applied twice. EMBO J 3: 2613–2619

    Google Scholar 

  34. Ryu KH, Park WM (1994) Construction of complementary DNA library and cDNA cloning for Cy strain of odontoglossum ringspot virus genomic RNA. Korean J Plant Pathol 10: 228–234

    Google Scholar 

  35. Ryu KH, Choi CW, Choi JK, Park WM (1995) Cloning of the 3′-terminal region encoding movement and coat proteins of a Korean isolate of odontoglossum ringspot virus. Arch Virol 140: 481–490

    Google Scholar 

  36. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467

    Google Scholar 

  37. Sleat DE, Gallie DR, Jefferson RA, Bevan MW, Turner PC, Wilson TMA (1987) Characterization of the 5′-leader sequence of tobacco mosaic virus RNA as a general enhancer of translationin vitro. Gene 217: 217–225

    Google Scholar 

  38. Solis I, Garcia-Arenal F (1990) The complete nucleotide sequence of the genomic RNA of the tobamovirus tobacco mild green mosaic virus. Virology 177: 553–558

    Google Scholar 

  39. Sulzinsky MA, Gabard KA, Palukaitis P, Zaitlin M (1985) Replication of tobacco mosaic virus. VIII. Characterization of a third subgenomic TMV RNA. Virology 145: 132–140

    Google Scholar 

  40. Tyc K, Kornaska M, Gross HJ, Filipowicz W (1984) Multiple ribosome binding to the 5′-terminal leader sequence of tobacco mosaic virus RNA, assembly of an 80S ribosome mRNA complex at the AUU codon. Eur J Biochem 140: 503–511

    Google Scholar 

  41. Ugaki M, Tomiyama M, Kakutani T, Hidaka S, Kiguchi T, Nagata R, Sato T, Motoyoshi F, Nishiguchi M (1991) The complete nucleotide sequence of cucumber green mottle mosaic virus (SH strain) genomic RNA. J Gen Virol 72: 1487–1495

    Google Scholar 

  42. Valverde RA, Dodds JA, Heick JA (1986) Double-stranded ribonucleic acid from plants infected with viruses having elongated particles and undivided genomes. Phytopathology 76: 459–465

    Google Scholar 

  43. Zettler FW, Ko NJ, Wisler GC, Elliot MS, Wong SM (1990) Viruses of orchids and their control. Plant Dis 74: 621–626

    Google Scholar 

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Authors and Affiliations

  1. Department of Agricultural Biology, College of Natural Resources, Korea University, Seoul, Korea

    K. H. Ryu & W. M. Park

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  1. K. H. Ryu
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  2. W. M. Park
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Additional information

The nucleotide sequence reported in this paper will appear in the EMBL, GenBank and DDBJ nucleotide sequence databases under the accession number X82130.

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Ryu, K.H., Park, W.M. The complete nucleotide sequence and genome organization of odontoglossum ringspot tobamovirus RNA. Archives of Virology 140, 1577–1587 (1995). https://doi.org/10.1007/BF01322531

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  • DOI: https://doi.org/10.1007/BF01322531

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